Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

ABSTRACT: Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from over 1,700 primary mouse dendritic cells (DCs) stimulated with three pathogenic components and examine variation between individual cells exposed to the same stimulus and general strategies that multicellular populations use to establish complex dynamic responses. RNA seq libraries from 1775 single cells and population controls with SMARTer

ORGANISM(S): Mus musculus

SUBMITTER: Rahul Satija

PROVIDER: E-GEOD-48968 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation.

Shalek Alex K AK Satija Rahul R Shuga Joe J Trombetta John J JJ Gennert Dave D Lu Diana D Chen Peilin P Gertner Rona S RS Gaublomme Jellert T JT Yosef Nir N Schwartz Schraga S Fowler Brian B Weaver Suzanne S Wang Jing J Wang Xiaohui X Ding Ruihua R Raychowdhury Raktima R Friedman Nir N Hacohen Nir N Park Hongkun H May Andrew P AP Regev Aviv A

Nature 20140611 7505

High-throughput single-cell transcriptomics offers an unbiased approach for understanding the extent, basis and function of gene expression variation between seemingly identical cells. Here we sequence single-cell RNA-seq libraries prepared from over 1,700 primary mouse bone-marrow-derived dendritic cells spanning several experimental conditions. We find substantial variation between identically stimulated dendritic cells, in both the fraction of cells detectably expressing a given messenger RNA ...[more]

PMID: 24919153

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Dataset Information

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

Publications

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation.

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