Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Tif1Beta-Hp1 system maintains transcriptional integrity of hematopoietic stem cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Satoru Miyagi

PROVIDER: E-GEOD-48987 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Gene expression changes during hematopoietic cell differentiation

Project description:Gene expression analyses of hematopoietic stem cells (HSCs), progenitor cells (HPCs), and differentiated cell. Gene expressions of long-term HSCs (CD34-ckit+Sca1+Lineage-), short term HSCs (CD34+ckit+Sca1+Lineage-), Progenitor cells (ckit+Sca1- Lineage-), and differentiated cels (Lineage+) were examined by microarray. Results provide insight into the mechanism of hematopoietic cell differentiation. Long-term HSCs (CD34-ckit+Sca1+Lineage-), Short term-HSCs (CD34+ckit+Sca1+Lineage-), Progenitor cells (ckit+Sca1- Lineage-, and Lineage+), and differentiated cell (Lineage+) were sorted from mouse bone marraw and were examined by microarray. Results provide insight into the mechanism of hematopoietic cell differentiation.

2013-11-06 | E-GEOD-48986 | biostudies-arrayexpress

RNA Sequencing of Gprc5c-Knockout (Gprc5c-KO) and wildtype (WT) control mice HSCs.

Project description:The transcriptome of Gprc5c-KO and WT control mice HSCs were assessed by RNA-seq.

2022-04-06 | E-MTAB-9862 | biostudies-arrayexpress

RNA-seq of mouse wildtype HSCs purified from recipient mice co-transplanted with wildtype or different types of lymphoid cell deficient HSCs

Project description:To investigate how HSCs functionally compensate for the B cell deficiencies of other HSCs within an organism, we co-transplanted wildtype (WT) HSCs and lineage-deficient HSCs into lethally irradiated WT recipient mice. WT HSCs were then purified from the bone marrow of recipient mice for RNA isolation and sequencing. The purpose is to determine whether there are common genes shared between compensating WT HSCs in the WT co-transplanted with uMT-/- (B6.129S2(B6)-Ighmtm1Cgn/J) and WT co-transplanted with NSG (NOD-scid IL2Rgamma null).

2018-06-14 | E-MTAB-6776 | biostudies-arrayexpress

Imprinting Affy

Project description:Murine long-term hematopoietic stem cells (HSCs), short-term HSCs and multipotent progenitor cells (MPPs) were isolated from bone marrow and expression profiled on Affy chips. The behavior of maternal-specific imprinting genes, particularly in the H19-Igf2 locus, was focused on, to see if any might be involved in maintaining quiescence of long-term stem cells.

2013-08-12 | E-MTAB-1644 | biostudies-arrayexpress

RNA-seq of long-term hematopoietic stem cells and myeloid progenitors of control mice or mice conditionally deficient for Trp53 or Elp3 or both genes in the hematopoietic system

Project description:Aim of the experiment was to compare the gene expression responses of long-term hematopoietic stem cells (HSCs) and myeloid-restricted progenitors (MyPs) to the hematopoietic cell-restricted deletion of the catalytic subunit Elp3 of the Elongator complex. Since Elp3 deletion resulted in activation of the p53 pathway, we also studied the transcriptome of MyPs deficient for Trp53 or for both Elp3 and Trp53. The Elp3fl/fl strain was generated in house and first described in (DOI: 10.1084/jem.20142288). The Trp53 strain was first described in (DOI: 10.1101/gad.14.8.994) and was purchased from the Jackson Laboratory. Littermates of 8-12 weeks old were used in all experiments.

2020-12-10 | E-MTAB-6992 | biostudies-arrayexpress

Gene profiling of HSCs in C57BL/6 TEL-SYK BM versus control

Project description:The aim of the study was to analyse gene expression differences in LT and ST HSCs in BM of TEL-SYK transplanted versus control C57BL/6mice

2014-12-31 | E-MTAB-2240 | biostudies-arrayexpress

Gene expression signatures in Tif1b-deficient hematopoietic stem cell

Project description:Gene expression analyses of Tif1b-deficient hematopoietic stem cells (HSCs). Tif1b loss leads to rapid depletion of HSCs. Expression analyses provide insight into the role of Tif1b in the maitenance of HSC. HSCs (c-Kit+ Sca1+ Lineage- ) are sorted from fetal liver or adult bone marrow and are subjected into microarray analyses.

2013-11-06 | E-GEOD-48929 | biostudies-arrayexpress

Transcription factor SOX17 overexpression in hematopoietic stem cells

Project description:This SuperSeries is composed of the following subset Series: GSE30444: Retroviral Sox17 over-expression adult hematopoietic stem/progenitor cells microarray GSE30445: Sox17-transgenic hematopoietic stem cell microarray Refer to individual Series

2011-07-14 | E-GEOD-30446 | biostudies-arrayexpress

Gene expression signatures in Tif1b, Hp1a or Hp1g knockdown hematopoietic progenitors

Project description:Gene expression analyses of Tif1b, Hp1a or Hp1g knockdown hematopoietic progenitors. Growth of hematopoietic stem cells (HSCs) are significantly impaired upon knockdown of Hp1a and Hp1g. Results provide insight into the role of these factors in hematopoiesis. HSCs (CD34- c-Kit+ Sca1+ Lineage- ) were transduced with lentivirus expressing shRNA against Tif1b, Hp1a or Hp1g, and cultured for 14 days. Then, GFP+ c-Kit+ Lineage- hematopoietic progenitors were sorted and subjected into microarray analysis

2014-08-01 | E-GEOD-48897 | biostudies-arrayexpress

Transcription profiling of mouse hematopoetic progenitor cells with Flk12+ Ncad-, Flk12- Ncad+, Flk12+ Ncad+ or Flk12- Ncad- phenotypes

Project description:N-cadherin expression level distinguishes reserved versus primed states of hematopoietic stem cells

2008-01-04 | E-TABM-404 | biostudies-arrayexpress