Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse atrioventricular junction gene expression at embryonic day 10.5-11.0

ABSTRACT: Goals of this study were to identify new candidates involved in the development of the Atrioventricular cushions in the mouse heart. Experiment Overall Design: The atrioventricular junction and ventricular region were dissected from 80 embryonic day 10.5-11.0, wild type, C57BL/6 mouse hearts. RNA was isolated from the respective regions, amplified using the Arcturus RiboAmp HS RNA amplification kit, and hybridized to Affymetrix MOE430A expression chips in duplicate. Comparison between atrioventricular gene expression and ventricular gene expression will allow for the identification of genes necessary in the development of the AV cushions.

ORGANISM(S): Mus musculus

SUBMITTER: Andy Wessels

PROVIDER: E-GEOD-4903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development.

Wirrig Elaine E EE Snarr Brian S BS Chintalapudi Mastan R MR O'neal Jessica L JL Phelps Aimee L AL Barth Jeremy L JL Fresco Victor M VM Kern Christine B CB Mjaatvedt Corey H CH Toole Bryan P BP Hoffman Stanley S Trusk Thomas C TC Argraves W Scott WS Wessels Andy A

Developmental biology 20070809 2

To expand our insight into cardiac development, a comparative DNA microarray analysis was performed using tissues from the atrioventricular junction (AVJ) and ventricular chambers of mouse hearts at embryonic day (ED) 10.5-11.0. This comparison revealed differential expression of approximately 200 genes, including cartilage link protein 1 (Crtl1). Crtl1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Immunohi ...[more]

PMID: 17822691

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Project description:E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells; In the study we demonstrated shared gene expression in embryonic heart valve development and Osteoblast progenitor cells. The atrioventricular (AV) valves of the heart develop from undifferentiated mesenchymal endocardial cushions, that later remodel into stratified valves with diversified extracellular matrix (ECM). Because the mature valves express genes associated with osteogenesis and exhibit disease-associated calcification, we hypothesized the existence of shared regulatory pathways active in the remodeling AV valves and in bone progenitor cells. In order to define gene regulatory programs of valvulogenesis relative to osteoblast progenitors, we undertook Affymetrix gene expression profiling analysis of murine embryonic day (E)12.5 AV cushions compared to E17.5 remodeled AV valves (mitral and tri-cuspid) and to pre-osteoblast MC3T3-E1 (subclone4) cells. Overall MC3T3 cells were significantly more similar to E17.5 valves than to E12.5 cushions, supporting the hypothesis that valve remodeling involves the expression of many genes also expressed in osteoblasts. Several transcription factors characteristic of mesenchymal and osteoblast precursor cells, including Twist1 are predominant in E12.5 cushion. Valve remodeling also includes differential regulation of matrix metalloproteinases and their inhibitors as well as characteristic collagen isoform switching. Among the most highly enriched genes during valvulogenesis were members of the small leucine-rich proteoglycan (SLRP) family including Asporin, a known negative regulator of osteoblast differentiation and mineralization. Together, these data support shared gene expression profiles of the remodeling valves and osteoblast bone precursor cells in normal valve development and homeostasis with potential functions in calcific valve disease. Experiment Overall Design: In the study, we hybridized RNA from E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells to Affymetrix MOE430 2 GeneChipÂ® arrays.

Dataset Information

Transcription profiling of mouse atrioventricular junction gene expression at embryonic day 10.5-11.0

Publications

Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development.

Similar Datasets

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