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Nucleosomes are context-specific, H2A.Z modulated barriers to RNA polymerase

ABSTRACT: Nucleosomes are barriers to transcription in vitro, however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA Polymerase II (RNAPII) at nucleotide resolution. Our results suggest that nucleosomes present significant, context-specific barriers to RNAPII in vivo that can be tuned by the incorporation of H2A.Z. 8 sets of two replicates each of paired-end and 3 sets of two replicates each of single-end samples were sequenced and analyzed.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jorja Henikoff

PROVIDER: E-GEOD-49106 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nucleosomes are context-specific, H2A.Z-modulated barriers to RNA polymerase.

Weber Christopher M CM Ramachandran Srinivas S Henikoff Steven S

Molecular cell 20140301 5

Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first (+1) nucleosome is a barrier to RNAPII for essentially all genes, including those undergoing regulated pausing farther upstream. In contrast to the +1 nucleosome, gene body nucleoso ...[more]

PMID: 24606920

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