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The induced APL cells generated by the transplantation of PML-RARA-transduced human CD34+ hematopoietic cells into immunodeficient mice

ABSTRACT: A humanized in vivo APL model has been established utilizing the retroviral transduction of PML-RARA into human CD34+ hematopoietic cells and the transplantation of these cells into immunodeficient mice. The resultant leukemia recapitulated human APL phenotypically, and was clustered in the same category as human APL samples in the gene expression analysis. CD34+ cells from human cord blood were retrovirally transduced with PML-RARA. They were then intravenously injected into a tail vein of nine- to 20-week-old NOD/Shi-SCID/IL-2Rγnull (NOG) mice which were irradiated with 220 cGy of X-rays. Three to four months later, the CD45+/ CD33+/ EGFP+ cells were sorted from the bone marrow for a microarray analysis. Two human APL/M3 AML samples were utilized for a comparison with these cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Hiromichi Matsushita 

PROVIDER: E-GEOD-49344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Establishment of a humanized APL model via the transplantation of PML-RARA-transduced human common myeloid progenitors into immunodeficient mice.

Matsushita Hiromichi H   Yahata Takashi T   Sheng Yin Y   Nakamura Yoshihiko Y   Muguruma Yukari Y   Matsuzawa Hideyuki H   Tanaka Masayuki M   Hayashi Hideki H   Sato Tadayuki T   Damdinsuren Anar A   Onizuka Makoto M   Ito Mamoru M   Miyachi Hayato H   Pandolfi Pier Paolo PP   Ando Kiyoshi K  

PloS one 20141104 11

Recent advances in cancer biology have revealed that many malignancies possess a hierarchal system, and leukemic stem cells (LSC) or leukemia-initiating cells (LIC) appear to be obligatory for disease progression. Acute promyelocytic leukemia (APL), a subtype of acute myeloid leukemia characterized by the formation of a PML-RARα fusion protein, leads to the accumulation of abnormal promyelocytes. In order to understand the precise mechanisms involved in human APL leukemogenesis, we established a  ...[more]

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