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Comparative transcriptional analysis of Desulfovibrio alaskensis G20, a choline utilization regulator and LysX

ABSTRACT: Expression data for Desulfovibrio alaskensis strain G20 and mutants in regulator proteins grown on lactate sulfate media and then pelleted and transferred to another media when they reached stationary phase. The Choline mutant was transferred to lacte/sulfate minimal media and choline/sulfate minimal media. The LysX mutant was transferred to minimal media with lysine and rich media. G20 was transferred to minimal media, choline/sulfate minimal media, lactate/choline/sulfate minimal media, minimal media with lysine, and rich media. We aimed to confirm or expand the regulons of each of the transposon interupted regulator mutants and compare gene expression responses of the regulators in different growth conditions. 10 samples were collected: 2 regulator mutants (2 conditions each), Desulfovibrio alaskensis G20 (5 conditions), 2 replicates for G20 minimal media condition. Control sample -G20 rich media.

ORGANISM(S): Desulfovibrio alaskensis G20

SUBMITTER: Jennifer Kuehl

PROVIDER: E-GEOD-49528 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparative transcriptional analysis of Desulfovibrio alaskensis G20, a choline utilization regulator and LysX

Project description:Expression data for Desulfovibrio alaskensis strain G20 and mutants in regulator proteins grown on lactate sulfate media and then pelleted and transferred to another media when they reached stationary phase. The Choline mutant was transferred to lacte/sulfate minimal media and choline/sulfate minimal media. The LysX mutant was transferred to minimal media with lysine and rich media. G20 was transferred to minimal media, choline/sulfate minimal media, lactate/choline/sulfate minimal media, minimal media with lysine, and rich media. We aimed to confirm or expand the regulons of each of the transposon interupted regulator mutants and compare gene expression responses of the regulators in different growth conditions.

2013-08-03 | GSE49528 | GEO

Comparative transcriptional analysis of Desulfovibrio alaskensis G20 and mutants in regulator proteins grown in minimal media

Project description:Expression data for Desulfovibrio alaskensis strain G20 and mutants in regulator proteins grown on lactate sulfate media. Pellets collected at mid-log growth phase. We aimed to confirm or expand the predicted regulons of each of the transposon interrupted regulator mutants. 8 samples were collected: 7 regulator mutants and 1 control sample Desulfovibrio alaskensis G20. 1 replicate for each condition.

2013-08-03 | E-GEOD-49527 | biostudies-arrayexpress

Comparative transcriptional analysis of Desulfovibrio alaskensis G20 and regulator proteins mutants

Project description:Expression data for Desulfovibrio alaskensis strain G20 and mutants in regulator proteins grown on lactate sulfate media. Pellets collected at mid-log growth phase. We aimed to confirm or expand the predicted regulons of each of the transposon interrupted regulator mutants. 12 samples were collected: 10 regulator mutants, 1 mutant in a secretory protein and 1 control sample Desulfovibrio alaskensis G20. 1 replicate for each condition.

2013-08-03 | E-GEOD-49529 | biostudies-arrayexpress

Influence of growth rate and methanogenic partner on syntrophic metabolism of Desulfovibrio G20 (comparative transcriptional analysis of Desulfovibrio G20 grown under various syntrophic conditions relative to respiratory growth)

Project description:Expression data for Desulfovibrio alaskensis strain G20 grown on lactate in sulfate-limited monoculture and syntrophic coculture with Methanococcus maripaludis or Methanospirillum hungatei in chemostats at a low growth rate of 0.027h-1. 7 samples of Desulfovibrio alaskensis strain G20 grown in syntrophic coculture on lactate with either Methanococcus maripaludis (4 replicates) or Methanospirillum hungatei (3 replicates), and 5 samples of sulfate-limited monoculture growth of strain G20 on lactate.

2013-05-02 | E-GEOD-46547 | biostudies-arrayexpress

Comparative transcriptional analysis of Desulfovibrio G20 grown under syntrophic and respiratory condtions

2012-11-29 | E-GEOD-42571 | biostudies-arrayexpress

Comparative transcriptional analysis of Desulfovibrio alaskensis G20 and mutants

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-03 | E-GEOD-49530 | biostudies-arrayexpress

Identification of transcript starts in Desulfovibrio alaskensis G20

Project description:5' RNASeq of mRNA from G20 in defined lactate/sulfate media (LS4D)

2013-08-03 | GSE49484 | GEO

Sulfate_Pyruvate_Desulfovibrio_G20

Project description:In order to genetically determine the essential components of electron flow during respiration of sulfate by the anaerobe Desulfovibrio desulfuricans G20, a growth mode independent of sulfate is needed. However, a mutant of the G20 strain, I2, lacking the abundant type-1 tetraheme cytochrome c3 (TpI-c3), was found to be altered in growth with sulfate. Here we report that I2 does not reduce sulfate with electrons from pyruvate. In contrast, in the absence of sulfate, fermentative growth of I2 on pyruvate actually yielded slightly greater cellular protein than G20. When provided lactate with sulfate or when fermenting pyruvate, I2 produced more hydrogen and accumulated little if any succinate. G20 grows by fumarate dismutation and accumulates the theoretical 2:1 ratio of the end products, succinate and acetate, respectively. In contrast, I2 did not grow on fumarate alone. We inferred that TpI-c3 might be required for transfer of electrons to the membrane-bound fumarate reductase or for establishing a redox environment needed to trigger synthesis of the enzymes for succinate production. Microarray and proteomic analyses of gene and protein expression in I2 compared with G20 were consistent with increases in those enzymes predicted to be necessary for the generation of end products measured. These results support a role for the periplasmic TpI-c3 in electron flow from pyruvate to sulfate and in establishing conditions for enzyme production for fumarate dismutation. In addition, evidence for the simultaneous operation of respiratory electron flow and substrate-level phosphorylation was obtained from end product analysis. Strains I2 and G20 of Desulfovibrio desulfuricans were grown in different media. Four biological replicates of G20 on lactate media, and two biological replicates of G20 on Pyruvate media were compared with three replicates of I2 on lactate media and two replicates of I2 on pyruvate media.

2010-04-25 | E-GEOD-21287 | biostudies-arrayexpress

High-resolution analysis of RNA expression in Desulfovbrio alaskensis G20

Project description:High-resolution tiling analysis of the D. alaskensis G20 transcriptome in lactate/sulfate media, both rich (LS) and defined (LS4D)

2012-07-19 | GSE39471 | GEO

Comparative transcriptional analysis of Desulfovibrio alaskensis G20 grown under various syntrophic and fermentative conditions on pyruvate relative to respiratory growth

Project description:Pyruvate fermentation pathway and energetics of Desulfovibrio alaskensis strain G20 under syntrophic coculture and fermentative monoculture conditions Expression data for Desulfovibrio alaskensis strain G20 grown in chemostats on pyruvate under respiratory conditions (sulfate-limited and pyruvate-limited monoculture, dilution rate 0.047 and 0.027 h-1), fermentative conditions (monoculture, dilution rate 0.036 h-1), and syntrophic conditions (coculture with Methanococcus maripaludis or Methanospirillum hungatei, dilution rate of 0.047 and 0.027 h-1) 2 replicates each for syntrophic coculture (M. maripaludis or M. hungatei pairing) and respiratory (sulfate- or pyruvate-limited) monoculture for both growth rates (0.027 and 0.047 h-1), and 4 replicates fermentative monoculture (gas flow rate through head space of bioreactor 10 ml/min (chemostats C91 and C93) or 1 ml/min (chemostats C92 and C94)

2013-12-12 | E-GEOD-53226 | biostudies-arrayexpress

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