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Evaluation of technical (array-to-array) reproducibility of L. vannamei microarray

ABSTRACT: Five aliquots of the same RNA sample were labeled and hybridized in parallel to the L. vannamei microarray, in order to assess the technical reproducibility of the tool Keywords: technical validation A sample of gill total RNA was divided in 5 identical aliquots, and each aliquot was labeled and hybridized to an array. The co-relation among the global expression profiles obtained was assessed.

ORGANISM(S): Litopenaeus vannamei

SUBMITTER: Yian Chen

PROVIDER: E-GEOD-4954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Insights into the immune transcriptome of the shrimp Litopenaeus vannamei: tissue-specific expression profiles and transcriptomic responses to immune challenge.

Robalino Javier J Almeida Jonas S JS McKillen David D Colglazier Joan J Trent Harold F HF Chen Yian Ann YA Peck Megan E T ME Browdy Craig L CL Chapman Robert W RW Warr Gregory W GW Gross Paul S PS

Physiological genomics 20061205 1

Infectious disease constitutes a major obstacle to the sustainability of shrimp aquaculture worldwide and a significant threat to natural populations of shrimp and other crustacea. The study of the shrimp immune system, including the response to viral infection, has been hampered by a relative lack of molecular genetic information and of tools suitable for high-throughput assessment of gene expression. In this report, the generation of a cDNA microarray encompassing 2,469 putative unigenes expre ...[more]

PMID: 17148689

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