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Transcription profiling of rat liver samples treated with pregnenolone 16alpha-carbonitrile

ABSTRACT: Activation of the pregnane X receptor (NR1I2) by drugs and other xenobiotics stimulates (or suppresses) expression of numerous genes involved in the metabolic elimination of foreign compounds and some toxic endogenous substrates. We used microarray analysis to identify genes whose expression in rat liver was significantly altered by pregnenolone 16alpha-carbonitrile (PCN) treatment. Experiment Overall Design: We chose to profile the gene expression pattern in the livers of adult rats to test the idea that PCN counteracts a wide variety of stresses by activating genes apart from drug metabolism.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Linda Quattrochi

PROVIDER: E-GEOD-4959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes controlled by the pregnane X receptor by microarray analysis of mRNAs from pregnenolone 16alpha-carbonitrile-treated rats.

Guzelian Jeffrey J Barwick Joyce L JL Hunter Lawrence L Phang Tzu L TL Quattrochi Linda C LC Guzelian Philip S PS

Toxicological sciences : an official journal of the Society of Toxicology 20060922 2

Mammalian liver contains a pregnane X receptor (PXR, NR1I2), which binds drugs and other xenobiotics, and stimulates (or suppresses) expression of numerous genes involved in the metabolic elimination of foreign compounds and some toxic endogenous substances. In the present study, we used microarray analysis to identify genes whose expression in rat liver was significantly altered by pregnenolone 16alpha-carbonitrile (PCN) treatment. PCN is a synthetic steroid that induces cytochrome P4503A expre ...[more]

PMID: 16997903

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