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Tissue specific expression of chalcone synthase siRNAs


ABSTRACT: In a previous study, seed coat and cotyledon tissues of Williams, Richland and T157 soybean lines were investigated to show tissue specificity of CHS siRNA expression (Tuteja et al., 2009). Here, we investigated more tissues such as leaf, root and germinating cotyledon to ascertain the tissue specificity of CHS siRNAs in Williams. Data from multiple small RNA libraries were sequenced deeply by the Illumina high-throughput sequencing technology. The total numbers of small RNA reads were from three million to thirty million, providing sufficient data to show the tissue specificity of CHS siRNA. High-throughput sequencing using Genome Analyzer II and Illumina HiSeq 2000 was performed.

ORGANISM(S): Glycine max

SUBMITTER: Lila Vodkin 

PROVIDER: E-GEOD-49708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The transition from primary siRNAs to amplified secondary siRNAs that regulate chalcone synthase during development of Glycine max seed coats.

Cho Young B YB   Jones Sarah I SI   Vodkin Lila L  

PloS one 20131021 10


The I locus is a 27-kb inverted repeat cluster of chalcone synthase genes CHS1-3-4 that mediates siRNA down-regulation of CHS7 and CHS8 target mRNAs during seed development leading to yellow seed coats lacking anthocyanin pigments. Here, we report small RNA sequencing of ten stages of seed development from a few days post fertilization through maturity, revealing the amplification from primary to secondary short interfering RNAs (siRNAs) occurring during development. The young seed populations h  ...[more]

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