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Expression data from additional fertilization rice seeds


ABSTRACT: Carbon (C) and nitrogen (N) contents of grain-filling stage are keys item that determined the growth of rice, and also the quality of seed. Therefore, to elucidating the mechanism of C/N signaling in a seed is an important problem for crops whose seed is used as food like rice. The DNA microarray analysis with the rice seed which was performed the additional fertilizer at the time of heading, in order to clarify how C/N signal change of the rhizosphere in seed production stage affects a seed component on a gene expression level. Fertilization was supplied at the same time of plantation and performed middle fertilizing 37 days after germination. 400 mg of ammonium chloride (NH4 Cl) was supplied at the time of heading to the “ + NH4 Cl ” group. Rice seeds were selected from 6 plants for RNA extraction, and hybridization on Affymetrix microarrays. We sought to understand the change of gene expression by the additional fertilization.

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Keiko Midorikawa 

PROVIDER: E-GEOD-49818 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Additional nitrogen fertilization at heading time of rice down-regulates cellulose synthesis in seed endosperm.

Midorikawa Keiko K   Kuroda Masaharu M   Terauchi Kaede K   Hoshi Masako M   Ikenaga Sachiko S   Ishimaru Yoshiro Y   Abe Keiko K   Asakura Tomiko T  

PloS one 20140606 6


The balance between carbon and nitrogen is a key determinant of seed storage components, and thus, is of great importance to rice and other seed-based food crops. To clarify the influence of the rhizosphere carbon/nitrogen balance during the maturation stage of several seed components, transcriptome analysis was performed on the seeds from rice plants that were provided additional nitrogen fertilization at heading time. As a result, it was assessed that genes associated with molecular processes  ...[more]

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