Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of deletants of two transcription factor B in the hyperthermophilic archaeon, Thermococcus kodakarensis.


ABSTRACT: Thermococcus kodakarensis, which can grow in a wide range of temperatures, from 60M-BM-0C to 100M-BM-0C, optimally at 85M-BM-0C, has two transcription factor Bs, TFB1 and TFB2, both of which are basal transcription factor. TFB1 is unique to hyperthermophiles, but TFB2 is widely conserved in Euryarchaeota. To screen the genes dependent on each TFB in a temperature dependent manner, we investigated the transcriptional profilings of each TFB deletant strain grown at each temperature, 60M-KM-^ZC, 85M-KM-^ZC, and 93M-KM-^ZC, by comparing with the host strain, KU216. Data analyses showed that TFB1 apparently controls the expression of genes essential for motility and adhesion, such as flagellin genes, at 85M-KM-^ZC and 93M-KM-^ZC, but TFB2 regulates genes involved in basal metabolism including amino acid biosynthesis. Two-condition experiment, KU216 vs. DTF1 cells and KU216 vs. DTF2. Technical replicates: 2 DTF1 at 60M-KM-^ZC, 2 DTF1 at 85M-KM-^ZC, 2 DTF1 at 93M-KM-^ZC, 2 DTF2 at 60M-KM-^ZC, 2 DTF2 at 85M-KM-^ZC, 2 DTF2 at 93M-KM-^ZC, independently measured. One replicate per array.

ORGANISM(S): Thermococcus kodakarensis

SUBMITTER: Ryota Hidese 

PROVIDER: E-GEOD-49841 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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