Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin organization in NPP-13 knockdown C. elegans embryos [seq]


ABSTRACT: C. elegans nuclear pore protein NPP-13 associates with small RNA genes transcribed by RNA Polymerase III. To test if the nuclear pore-chromatin interactions play a role in large-scale chromatin organization, we determined nuclear membrane-genome interactions and RNA Polymerase II localization in C. elegans embryos depleted for NPP-13. Genome-wide ChIP-seq and ChIP-chip for nuclear membrane protein LEM-2, RNA Polymerase II (AMA-1) and H3K4me3 were performed in mixed-stage C. elegans embryos depleted for NPP-13. As a control, ChIP was also performed in wild-type embryos treated with empty vector.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Kohta Ikegami 

PROVIDER: E-GEOD-49903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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