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Inducible overexpression analysis of KUODA1 in Arabidopsis seedlings

ABSTRACT: To identify potenital target genes of the transcription factor KUODA1 (AT5G47390), we performed expression profiling using an estradiol inducible overexpression system. Estradiol-treatment allows for the nuclear translocation of the constitutively expressed chimeric transcription factor XVE and subsequent expression of KUA1 from the LexA promoter. Stable trangenic T3 seedlings were grown for 14 days and either treated with 15 M-BM-5M estradiol or ethanol (mock) for 4 hours. Two mock and two estradiol treated samples were used for RNA isolation and hybridization.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jos Schippers

PROVIDER: E-GEOD-50520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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