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A role for H3K4 mono-methylation in facultative heterochromatin assembly and partitioning of chromatin readers

ABSTRACT: Mono-methylation of lysine 4 on histone H3 (H3K4me1) is a well-established feature of enhancers and promoters, although its function is unknown. Remarkably, we find H3K4me1 at the promoter regions of conditionaly-repressed muscle and stimulus-dependent inflammatory response genes in myoblasts. During myogenesis, muscle genes are activated and become H3K4-trimethylated. On the promoters of active genes, we find that H3K4me1 spatially restricts the recruitment of “readers” of H3K4me3, including ING1, which, in turn, recruits Sin3A. Our findings point to a unique role for H3K4 mono-methylation in establishing boundaries that restrict the recruitment of chromatin-modifying enzymes to defined regions within promoters. Mapping of Wdr5, Menin, MLL1, Sin3A, and LSD1 in growing myoblasts (MB) and ING1 in growing myoblasts (MB) and fully differentiated myotubes (MT).

ORGANISM(S): Mus musculus

SUBMITTER: Roy Blum

PROVIDER: E-GEOD-50590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A role for H3K4 monomethylation in gene repression and partitioning of chromatin readers.

Cheng Jemmie J Blum Roy R Bowman Christopher C Hu Deqing D Shilatifard Ali A Shen Steven S Dynlacht Brian D BD

Molecular cell 20140301 6

Monomethylation of lysine 4 on histone H3 (H3K4me1) is a well-established feature of enhancers and promoters, although its function is unknown. Here, we uncover roles for H3K4me1 in diverse cell types. Remarkably, we find that MLL3/4 provokes monomethylation of promoter regions and the conditional repression of muscle and inflammatory response genes in myoblasts. During myogenesis, muscle genes are activated, lose MLL3 occupancy, and become H3K4-trimethylated through an alternative COMPASS compl ...[more]

PMID: 24656132

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