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Identification of genes expressed with temporal-spatial restriction to developing cerebellar neuron precursors by a functional genomic approach perspective of human cancers.

ABSTRACT: Swiss-Webster B mouse postnatal day 4-5 primary cerebellar culture (pooled from litter mates) treated with sonic hedgehog (Shh), controls (veh), growth arrested (arrest), cycloheximide (cyc) for 1, 3 and 24 hours. Different treatment conditions with biological replicates. Mouse cerebellar granule cell neuron precursors under different treatment conditions.

ORGANISM(S): Mus musculus

SUBMITTER: Alvin Kho

PROVIDER: E-GEOD-50606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes expressed with temporal-spatial restriction to developing cerebellar neuron precursors by a functional genomic approach.

Zhao Qing Q Kho Alvin A Kenney Anna Marie AM Yuk Di Dong-in DI Kohane Isaac I Rowitch David H DH

Proceedings of the National Academy of Sciences of the United States of America 20020401 8

Hedgehog pathway activation is required for proliferation of cerebellar granule cell neuron precursors during development and is etiologic in certain cerebellar tumors. To identify genes expressed specifically in granule cell neuron precursors, we used oligonucleotide microarrays to analyze regulation of 13,179 genes/expressed sequence tags in heterogeneous primary cultures of neonatal mouse cerebellum that respond to the mitogen Sonic hedgehog. In conjunction, we applied experiment-specific noi ...[more]

PMID: 11960025

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