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Transcriptional regulation patterns revealed by high-resolution chromatin immunoprecipitation during cardiac hypertrophy

ABSTRACT: Purpose: To identify the differential transcriptional patterns during postnatal cardiac growth, pressure-induced cardiac hypertrophy and adult mouse hearts Results: Our data revealed novel transcriptional patterns during cardiac growth conditions. The results showed that most of the essential genes are regulated by promoter clearance of paused RNA pol II, while de novo recruitment is required for regulation of mostly speclaized genes during cardiac growth. We have identified two major modes of gene regulation durign cardiac hypertrophy and four transcriptional growth patterns. The results are published in January 2013 issue of Journal of Biological Chemistry. Methods: Hearts were extracted from 1-2day old C57 mice, from mice subjecetd to Transaortic coarctation or adult mice. The hearts were sent to Active Motif for RNA polII and H3K9ac- ChIP-Seq.

ORGANISM(S): Mus musculus

SUBMITTER: Maha Abdellatif

PROVIDER: E-GEOD-50637 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional regulation patterns revealed by high resolution chromatin immunoprecipitation during cardiac hypertrophy.

Sayed Danish D He Minzhen M Yang Zhi Z Lin Lin L Abdellatif Maha M

The Journal of biological chemistry 20121210 4

Cardiac hypertrophy is characterized by a generalized increase in gene expression that is commensurate with the increase in myocyte size and mass, on which is superimposed more robust changes in the expression of specialized genes. Both transcriptional and posttranscriptional mechanisms play fundamental roles in these processes; however, genome-wide characterization of the transcriptional changes has not been investigated. Our goal was to identify the extent and modes, RNA polymerase II (pol II) ...[more]

PMID: 23229551

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