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Transcription profiling of rat at pheochromocytoma cell line PC12 treated with trimethyltin at low and high doses

ABSTRACT: The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 hours prior to TMT treatment. Gene expression patterns were then analysed using Rat Expression Array 230A Experiment Overall Design: In this study we analize gene expression patterns in PC12 cells treated with Trimethyltin (TMT). We utilized control cells (untreated) and two different concentration (1 and 5) Experiment Overall Design: We used three biological replicates, for the three concentration tested, according to MIAME guidelines Experiment Overall Design: (total 9 chips were used in this study).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Camilla Bernardini

PROVIDER: E-GEOD-5073 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hypoxia-like transcriptional activation in TMT-induced degeneration: microarray expression analysis on PC12 cells.

Lattanzi Wanda W Bernardini Camilla C Gangitano Carlo C Michetti Fabrizio F

Journal of neurochemistry 20070301 6

To more clearly elucidate the complete network of molecular mechanisms induced by trimethyltin (TMT) toxicity, we used a homogeneous cell culture model represented by PC12 cells treated with 1 and 5 micromol/L TMT for 24 h. The gene expression profile was performed by microarray analysis, enabling us to identify 189 genes that were significantly modulated in treated cells, compared with controls. The main effects of TMT on gene expression seem to be related to the activation of metabolic process ...[more]

PMID: 17348866

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