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Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity

ABSTRACT: These data include the genome wide location of different histone modifications by ChIP sequencing in mouse ES cells, and RNA Seq data generated from wild type and EED KO mouse ES cells and knocked down for unrelated protein and Setd2 protein. ChIP-Seq: Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type mouse E14 ES cells, wild type E36 ES cells, EED KO E36 ES cells, wild type Embryoid bodies (Ebs), EED KO Embryoid bodies (Ebs EED KO) using specific antibody against different histone modifications. RNA-Seq: Total RNA extracted from wild type E36 ES cells, EED KO E36 ES cells, wild type E36 Embryoid bodies (Ebs), EED KO Embryoid bodies (Ebs EED KO), E14 Ctrl KD, E14 Setd2 KD.

ORGANISM(S): Mus musculus

SUBMITTER: Sri Ganesh Jammula

PROVIDER: E-GEOD-51006 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity.

Ferrari Karin J KJ Scelfo Andrea A Jammula Sriganesh S Cuomo Alessandro A Barozzi Iros I Stützer Alexandra A Fischle Wolfgang W Bonaldi Tiziana T Pasini Diego D

Molecular cell 20131127 1

H3K27me3 is deposited at promoters by the preferential association of Polycomb repressive complex 2 (PRC2) with CpG-rich DNA elements regulating development by repressing gene transcription. H3K27 is also present in mono- and dimethylated states; however, the functional roles of H3K27me1 and H3K27me2 deposition remain poorly characterized. Here, we show that PRC2 activity is not only associated with H3K27me3 but also regulates all forms of H3K27 methylation in a spatially defined manner, contrib ...[more]

PMID: 24289921

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