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TALEN-based knockout of mir-141 and mir-200c in SK-BR-3 cells

ABSTRACT: We used transcription activator-like effector nucleases (TALENs) to generate knockout cells for two related microRNAs (miRNAs), mir-141 and mir-200c, which belong to the deeply conserved mir-200 family. By carrying out deep sequencing, we identified the target genes of each miRNA. Interestingly, miR-141 and miR-200c, despite their overall similarity, suppressed largely non-overlapping groups of targets. Analysis of global mRNA level change in mir-141 and mir-200c knockout compared to wild type cells

ORGANISM(S): Homo sapiens

SUBMITTER: Young-Kook Kim

PROVIDER: E-GEOD-51217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from differentiating ES cells expressing miR-200c and miR-141

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