Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

LNCaP prostate cancer cell lines overexpressing wild-type or GARRPR-mutant Bag-1L

ABSTRACT: The BF-3 pocket of the androgen receptor (AR) has been identified as an allosteric modulator of the transactivation function of the AR. We now demonstrate that a duplicated GARRPR motif at the N-terminus of the cochaperone Bag-1L functions through this BF-3 domain. Amino acid exchanges in these two motifs impair binding of Bag-1L to the AR but increase the androgen-dependent activation of a subset of AR-target genes. We have therefore identified GARRPR as a novel BF-3 regulatory sequence important for fine-tuning the activity of the receptor. LNCaP cells stably expressing the empty vector construct (=control), or wild-type or N-terminal GARRPR mutant Bag-1L were cultured under hormone-starvation conditions for 72 h and then treated with vehicle or 10 nM DHT for 4 h. Biological triplicate samples were analyzed for each cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Laura Cato 

PROVIDER: E-GEOD-51524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

The androgen receptor (AR) is a ligand-activated transcription factor that is essential for prostate cancer development. It is activated by androgens through its ligand-binding domain (LBD), which consists predominantly of 11 α-helices. Upon ligand binding, the last helix is reorganized to an agonist conformation termed activator function-2 (AF-2) for coactivator binding. Several coactivators bind to the AF-2 pocket through conserved LXXLL or FXXLF sequences to enhance the activity of the recept  ...[more]

Similar Datasets

Transcriptomics LNCaP prostate cancer cell lines overexpressing wild-type or GARRPR-mutant Bag-1L
2014-02-18 | GSE51524 | GEO
Transcriptomics Targeting the androgen receptor N-terminus via the cochaperone Bag-1L [RNA-seq C-terminal mutant]
2017-08-23 | GSE102183 | GEO
Genomics Targeting the androgen receptor N-terminus via the cochaperone Bag-1L [ChIP-Seq]
2017-08-23 | GSE89938 | GEO
Transcriptomics Targeting the androgen receptor N-terminus via the cochaperone Bag-1L [RNA-Seq KO]
2017-08-23 | GSE89917 | GEO
Proteomics Androgen receptor RIME in prostate cancer cells
2022-07-12 | PXD031273 | Pride
Genomics Redefinition of Human Androgen Responsive Elements [FOX_ChIP_exo]
2015-01-01 | E-GEOD-43678 | biostudies-arrayexpress
Genomics Effect of p53 knowkdown on genome-wide map of AR-DNA binding
2012-07-31 | E-GEOD-31294 | biostudies-arrayexpress
Transcriptomics Expression data from LNCaP cells treated with DHT and enzalutamide
2013-04-01 | E-GEOD-44905 | biostudies-arrayexpress
Transcriptomics Time course of androgen-regulated transcripts in LNCaP cells
2016-07-01 | E-GEOD-70150 | biostudies-arrayexpress
Transcriptomics Genome wide analysis of androgen-regulated miRNAs
2015-09-01 | E-GEOD-66035 | biostudies-arrayexpress