Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of mouse laser captured small intestine isolated fom normal and germ free animals


ABSTRACT: This study delineated how small intestinal resident microflora impact gene expression in Paneth cells. Experiment Overall Design: Paneth cells were isolated by laser capture microdissection from the small intestines of germ-free and conventionalized (10 day) mice. RNAs from 3 mice per group were pooled, and duplicate RNAs from each group were amplified and hybridized to Affymetrix arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Lora Hooper 

PROVIDER: E-GEOD-5156 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Symbiotic bacteria direct expression of an intestinal bactericidal lectin.

Cash Heather L HL   Whitham Cecilia V CV   Behrendt Cassie L CL   Hooper Lora V LV  

Science (New York, N.Y.) 20060801 5790


The mammalian intestine harbors complex societies of beneficial bacteria that are maintained in the lumen with minimal penetration of mucosal surfaces. Microbial colonization of germ-free mice triggers epithelial expression of RegIIIgamma, a secreted C-type lectin. RegIIIgamma binds intestinal bacteria but lacks the complement recruitment domains present in other microbe-binding mammalian C-type lectins. We show that RegIIIgamma and its human counterpart, HIP/PAP, are directly antimicrobial prot  ...[more]

Similar Datasets

2014-01-03 | E-MTAB-1281 | biostudies-arrayexpress
2006-11-18 | E-GEOD-6290 | biostudies-arrayexpress
2010-04-09 | E-TABM-957 | biostudies-arrayexpress
2010-04-12 | E-TABM-958 | biostudies-arrayexpress
2018-10-31 | E-MTAB-6168 | biostudies-arrayexpress
2014-07-07 | E-GEOD-57724 | biostudies-arrayexpress
2013-02-08 | E-GEOD-44101 | biostudies-arrayexpress
2014-07-01 | E-GEOD-58948 | biostudies-arrayexpress
2022-01-01 | E-MTAB-10038 | biostudies-arrayexpress
2011-12-31 | E-GEOD-26095 | biostudies-arrayexpress