Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Jakub Westholm
PROVIDER: E-GEOD-51585 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila.
Yang Jr-Shiuan JS Smibert Peter P Westholm Jakub O JO Jee David D Maurin Thomas T Lai Eric C EC
Nucleic acids research 20131112 3
Although Dicer is essential for general microRNA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent. Instead, its short pre-miRNA hairpin is 'sliced' by Ago2, then 3'-resected into mature miRNAs. Here, we show that Drosophila cells and animals generate functional small RNAs from mir-451-type precursors. However, their bulk maturation arrests as Ago-cleaved pre-miRNAs, which mostly associate with the RNAi effector AGO2. Routing of pre-mir-451 hairpins to the miRNA effector AGO1 was inhib ...[more]
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