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Identification of IL-21-induced STAT3 dependent genes in human B cells

ABSTRACT: IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3 We used microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner Sort-purified naÃ¯ve B cells from four healthy donors and three patients with AD-HIES due to heterozygous STAT3 mutations were cultured for four days with CD40L in the presence or absence of IL-21 (50ng/ml). After four days, RNA was extracted from the harvested cells and the genetic profile was analysed by microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: Stuart Tangye

PROVIDER: E-GEOD-51587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-21 signalling via STAT3 primes human naive B cells to respond to IL-2 to enhance their differentiation into plasmablasts.

Berglund Lucinda J LJ Avery Danielle T DT Ma Cindy S CS Moens Leen L Deenick Elissa K EK Bustamante Jacinta J Boisson-Dupuis Stephanie S Wong Melanie M Adelstein Stephen S Arkwright Peter D PD Bacchetta Rosa R Bezrodnik Liliana L Dadi Harjit H Roifman Chaim M CM Fulcher David A DA Ziegler John B JB Smart Joanne M JM Kobayashi Masao M Picard Capucine C Durandy Anne A Cook Matthew C MC Casanova Jean-Laurent JL Uzel Gulbu G Tangye Stuart G SG

Blood 20131024 24

B-cell responses are guided by the integration of signals through the B-cell receptor (BCR), CD40, and cytokine receptors. The common γ chain (γc)-binding cytokine interleukin (IL)-21 drives humoral immune responses via STAT3-dependent induction of transcription factors required for plasma cell generation. We investigated additional mechanisms by which IL-21/STAT3 signaling modulates human B-cell responses by studying patients with STAT3 mutations. IL-21 strongly induced CD25 (IL-2Rα) in normal, ...[more]

PMID: 24159173

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