Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Identification of IL-21-induced STAT3 dependent genes in human B cells


ABSTRACT: IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3 We used microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner Sort-purified naïve B cells from four healthy donors and three patients with AD-HIES due to heterozygous STAT3 mutations were cultured for four days with CD40L in the presence or absence of IL-21 (50ng/ml). After four days, RNA was extracted from the harvested cells and the genetic profile was analysed by microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: Stuart Tangye 

PROVIDER: E-GEOD-51587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


B-cell responses are guided by the integration of signals through the B-cell receptor (BCR), CD40, and cytokine receptors. The common γ chain (γc)-binding cytokine interleukin (IL)-21 drives humoral immune responses via STAT3-dependent induction of transcription factors required for plasma cell generation. We investigated additional mechanisms by which IL-21/STAT3 signaling modulates human B-cell responses by studying patients with STAT3 mutations. IL-21 strongly induced CD25 (IL-2Rα) in normal,  ...[more]

Similar Datasets

2014-03-06 | E-GEOD-55607 | biostudies-arrayexpress
2015-07-01 | E-GEOD-63204 | biostudies-arrayexpress
2015-07-01 | GSE63204 | GEO
2015-02-05 | E-GEOD-52798 | biostudies-arrayexpress
2018-05-04 | GSE109030 | GEO
2018-05-02 | GSE107719 | GEO
2013-03-26 | E-GEOD-45466 | biostudies-arrayexpress
2014-03-06 | GSE55607 | GEO
2010-01-08 | E-GEOD-19198 | biostudies-arrayexpress
2010-01-08 | GSE19198 | GEO