Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Real-time quantitative PCR analysis of hormone-receptor-negative primary breast tumors

ABSTRACT: Total RNA was extracted from FFPE samples of 139 chemotherapy-naÃ¯ve hormone-receptor-negative breast cancers pooled from three sample sources and assayed for the expression of 21 genes within two previously reported microarray-derived prognostic signatures in 6 RT-PCR multiplexes. RT-PCR gene expression profiling. Total RNA was extracted from 10 micron FFPE sections, and expression levels of the 21 genes of interest plus two reference genes were quantified with 6 multiplex RT-PCR TaqMan assays.

ORGANISM(S): Homo sapiens

SUBMITTER: Christina Yau

PROVIDER: E-GEOD-51630 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An optimized five-gene multi-platform predictor of hormone receptor negative and triple negative breast cancer metastatic risk.

Yau Christina C Sninsky John J Kwok Shirley S Wang Alice A Degnim Amy A Ingle James N JN Gillett Cheryl C Tutt Andrew A Waldman Fred F Moore Dan D Esserman Laura L Benz Christopher C CC

Breast cancer research : BCR 20130101 5

<h4>Introduction</h4>Outcome predictors in use today are prognostic only for hormone receptor-positive (HRpos) breast cancer. Although microarray-derived multigene predictors of hormone receptor-negative (HRneg) and/or triple negative (Tneg) breast cancer recurrence risk are emerging, to date none have been transferred to clinically suitable assay platforms (for example, RT-PCR) or validated against formalin-fixed paraffin-embedded (FFPE) HRneg/Tneg samples.<h4>Methods</h4>Multiplexed RT-PCR was ...[more]

PMID: 24172169

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Dataset Information

Real-time quantitative PCR analysis of hormone-receptor-negative primary breast tumors

Publications

An optimized five-gene multi-platform predictor of hormone receptor negative and triple negative breast cancer metastatic risk.

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