Unknown,Transcriptomics,Genomics,Proteomics

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EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics


ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ghanasyam Rallapalli 

PROVIDER: E-GEOD-51721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics.

Rallapalli Ghanasyam G   Kemen Eric M EM   Robert-Seilaniantz Alexandre A   Segonzac Cécile C   Etherington Graham J GJ   Sohn Kee Hoon KH   MacLean Daniel D   Jones Jonathan D G JD  

BMC genomics 20140506


<h4>Background</h4>Next Generation Sequencing technologies have facilitated differential gene expression analysis through RNA-seq and Tag-seq methods. RNA-seq has biases associated with transcript lengths, lacks uniform coverage of regions in mRNA and requires 10-20 times more reads than a typical Tag-seq. Most existing Tag-seq methods either have biases or not high throughput due to use of restriction enzymes or enzymatic manipulation of 5' ends of mRNA or use of RNA ligations.<h4>Results</h4>W  ...[more]

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