Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Ghanasyam Rallapalli
PROVIDER: E-GEOD-51721 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Rallapalli Ghanasyam G Kemen Eric M EM Robert-Seilaniantz Alexandre A Segonzac Cécile C Etherington Graham J GJ Sohn Kee Hoon KH MacLean Daniel D Jones Jonathan D G JD
BMC genomics 20140506
<h4>Background</h4>Next Generation Sequencing technologies have facilitated differential gene expression analysis through RNA-seq and Tag-seq methods. RNA-seq has biases associated with transcript lengths, lacks uniform coverage of regions in mRNA and requires 10-20 times more reads than a typical Tag-seq. Most existing Tag-seq methods either have biases or not high throughput due to use of restriction enzymes or enzymatic manipulation of 5' ends of mRNA or use of RNA ligations.<h4>Results</h4>W ...[more]