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Determining the number of individuals to pool

ABSTRACT: We compared 10, 20 and 40 animal pools to determine the best number of intact adults to pool Keywords: Agilent microarray, pooling, Drosophila, intact adults 16 vials of flies were reared. From each vial 5 individuals were selected. For the 10 animal comparison 4 vials were used with each replicate being a pool of 5 animals over 2 independent vials. For the 20 animal comparison 8 vials were used with each replicate being a pool of 5 animals over 5 independent vials and for the 40 animal comparison each replicate was a pool of 5 animals over 8 independent vials. There are two independent replicates of each type for 6 samples which were hybridized on three chips in a loop design.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Lauren McIntyre

PROVIDER: E-GEOD-5183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Simpler mode of inheritance of transcriptional variation in male Drosophila melanogaster.

Wayne Marta L ML Telonis-Scott Marina M Bono Lisa M LM Harshman Larry L Kopp Artyom A Nuzhdin Sergey V SV McIntyre Lauren M LM

Proceedings of the National Academy of Sciences of the United States of America 20071114 47

Sexual selection drives faster evolution in males. The X chromosome is potentially an important target for sexual selection, because hemizygosity in males permits accumulation of alleles, causing tradeoffs in fitness between sexes. Hemizygosity of the X could cause fundamentally different modes of inheritance between the sexes, with more additive variation in males and more nonadditive variation in females. Indeed, we find that genetic variation for the transcriptome is primarily additive in mal ...[more]

PMID: 18003923

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Determining the number of individuals to pool

Project description:We compared 10, 20 and 40 animal pools to determine the best number of intact adults to pool Keywords: Agilent microarray, pooling, Drosophila, intact adults

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Project description:We tested a new wash solution from Agilent Keywords: Agilent microarray, pooling, Drosophila, intact adults Intact males and females for a single genotype were examined. Two independent replicates of each were compared on two slides in a dye flip.

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