Unknown,Transcriptomics,Genomics,Proteomics

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H2AV_9751.S2.H2AV_RNAi.Solexa


ABSTRACT: modENCODE_submission_5595 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The goal of these experiments are to a) validate/confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome and b) evaluate their biological significance by assaying the impact of depletion on other proteins/marks. We are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, followed by Chromatin ImmunoPrecipitation (ChIP) assayed on genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody H2AV 9751 (target is H2AV); dsRNA (RNAi_reagent) CG5499_RNAi_2&oldid=76948

ORGANISM(S): Drosophila melanogaster

SUBMITTER: DCC modENCODE 

PROVIDER: E-GEOD-51990 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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