Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

5'RNA-seq analysis of hypertrophic cardiomyopathy (HCM) in mice that carry a human missence mutation in the myosin heavy chain

ABSTRACT: We developed a 5'RNA-seq methodology to concurrently assess gene expression and start-site usage changes. We applied this methodology to study hypertrophic cardiomyopathy in mice harboring a human deleterious mutation. 5'RNA-seq analysis of transcriptomes from mouse hearts with or without hypertrophic cardiomyopathy. Biological replicates were pooled into a single sequencing run. 5'RNA-seq methodology consists of enhanced sequencing of 5' ends and computational assessment of changes at start-sites of genes.

ORGANISM(S): Mus musculus

SUBMITTER: Jonathan Seidman

PROVIDER: E-GEOD-52038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

5'RNA-Seq identifies Fhl1 as a genetic modifier in cardiomyopathy.

Christodoulou Danos C DC Wakimoto Hiroko H Onoue Kenji K Eminaga Seda S Gorham Joshua M JM DePalma Steve R SR Herman Daniel S DS Teekakirikul Polakit P Conner David A DA McKean David M DM Domenighetti Andrea A AA Aboukhalil Anton A Chang Stephen S Srivastava Gyan G McDonough Barbara B De Jager Philip L PL Chen Ju J Bulyk Martha L ML Muehlschlegel Jochen D JD Seidman Christine E CE Seidman J G JG

The Journal of clinical investigation 20140210 3

The transcriptome is subject to multiple changes during pathogenesis, including the use of alternate 5' start-sites that can affect transcription levels and output. Current RNA sequencing techniques can assess mRNA levels, but do not robustly detect changes in 5' start-site use. Here, we developed a transcriptome sequencing strategy that detects genome-wide changes in start-site usage (5'RNA-Seq) and applied this methodology to identify regulatory events that occur in hypertrophic cardiomyopathy ...[more]

PMID: 24509080

Dataset Information

5'RNA-seq analysis of hypertrophic cardiomyopathy (HCM) in mice that carry a human missence mutation in the myosin heavy chain

Publications

5'RNA-Seq identifies Fhl1 as a genetic modifier in cardiomyopathy.

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