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Expression data from Col-0 and sp1,spx2 under phosphate starvation stress and recovery after resupplying phosphate

ABSTRACT: We performed a transcriptomic analysis of Pi-starvation and recovery after resupplying Pi in Arabidopsis thaliana (Columbia-0) wild type plants and double mutant spx1,spx2. Results show that SPX1 is a Pi-dependent inhibitor of the transcription factor PHR1, a central regulatory protein in the control of transcriptional responses to Pi starvation. The analysis was performed in wild-type and double spx1,spx2 double mutant plants grown for seven days in complete (+Pi) and Pi-lacking (-Pi) Johnson solid media. Then, plants were transferred to complete medium for 4 hours to analyze the effect of resupplying plants with Pi.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Enrique Rojo

PROVIDER: E-GEOD-52046 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SPX1 is a phosphate-dependent inhibitor of Phosphate Starvation Response 1 in Arabidopsis.

Puga María Isabel MI Mateos Isabel I Charukesi Rajulu R Wang Zhiye Z Franco-Zorrilla José M JM de Lorenzo Laura L Irigoyen María L ML Masiero Simona S Bustos Regla R Rodríguez José J Leyva Antonio A Rubio Vicente V Sommer Hans H Paz-Ares Javier J

Proceedings of the National Academy of Sciences of the United States of America 20140930 41

To cope with growth in low-phosphate (Pi) soils, plants have evolved adaptive responses that involve both developmental and metabolic changes. Phosphate Starvation Response 1 (PHR1) and related transcription factors play a central role in the control of Pi starvation responses (PSRs). How Pi levels control PHR1 activity, and thus PSRs, remains to be elucidated. Here, we identify a direct Pi-dependent inhibitor of PHR1 in Arabidopsis, SPX1, a nuclear protein that shares the SPX domain with yeast ...[more]

PMID: 25271326

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Expression data from Arabidopsis thaliana PHR1 primary targets genes under phosphate starvation stress

Project description:We performed a transcriptomic analysis of Pi starvation responses in Arabidopsis thaliana (Columbia-0) phr1 mutant plants expressing PHR1 in presence of cicloheximide, that inhibit protein translation, thus preventing any effect of PHR1 on the expression of indirect targets. Results show the primary target genes of PHR1 in the responses to Pi starvation. The analysis was performed in phr1 plants and phr1 plants overexpressing a fusion GR:PHR1 (OXGR:PHR1) whose activity is postranslationally controlled by dexamethasone (DEX), that allows studying gene expression upon PHR1 activation and the concomitant inhibition of translation with cycloheximide (CHX), thus preventing any effect of PHR1 on the expression of indirect targets. To perform this study, phr1 and OXGR:PHR1 phr1 plants were grown for seven days in complete Johnson liquid media (+Pi), 2 days in Pi-lacking media (-Pi) and then supplemented with 5 µM DEX and 10 µM CHX for 6 hours before harvesting. Three independent biological samples of total RNA from shoot and root were hybridized separately.

2010-12-09 | E-GEOD-20955 | biostudies-arrayexpress

Expression data from Col-0 and sp1,spx2 under phosphate starvation stress and recovery after resupplying phosphate

Project description:We performed a transcriptomic analysis of Pi-starvation and recovery after resupplying Pi in Arabidopsis thaliana (Columbia-0) wild type plants and double mutant spx1,spx2. Results show that SPX1 is a Pi-dependent inhibitor of the transcription factor PHR1, a central regulatory protein in the control of transcriptional responses to Pi starvation.

2014-09-30 | GSE52046 | GEO

Expression data from Arabidopsis thaliana under phosphate starvation stress

Project description:We performed a transcriptomic analysis of Pi starvation responses in Arabidopsis thaliana (Columbia-0) wild type plants under phosphate starvation stress and in plants with altered PHR1(-like) activity, comparing mutants of phr1 and phr1-phl1 grown in phosphate-lacking medium. Results show the central role of PHR1 and functionally redundant members of its family in the control of transcriptional responses to Pi starvation. The analysis was performed in wild-type plants grown for seven days in complete (+Pi) and Pi-lacking (-Pi) Johnson solid media and the single phr1 and double phr1-phl1 mutants grown for 7 days in –Pi medium. Three independent biological samples of total RNA from shoot and root were hybridized separately.

2010-12-09 | E-GEOD-16722 | biostudies-arrayexpress

Expression data from Arabidopsis thaliana under phosphate starvation stress

2010-12-09 | GSE16722 | GEO

Expression data from Arabidopsis thaliana PHR1 primary targets genes under phosphate starvation stress

2010-12-09 | GSE20955 | GEO

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Deubiquitinating Enzyme OTU5 Contributes to DNA Methylation Patterns and Is Critical for Phosphate Nutrition Signals

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2017-11-02 | GSE81407 | GEO

Whole Genome Bisulfite sequencing of Arabidopsis thaliana High and Low phosphate methylomes

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A specific protective mechanism against chloroplast photo-reactive oxygen species in phosphate-starved rice plants [ChIP-seq]

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