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Genome-wide maps of Tcf1 binding locations in splenic CD8 T cells.

ABSTRACT: We mapped genome-wide Tcf1 binding locations in mature CD8 T cells to identify its direct target genes. This experiment aims to identify genome-wide binding locations for Tcf1 transcription factor in mature CD8 T cells. The CD8 T cells were isolated from wild-type C57BL/6 mice by negative selection. The chromatin fragments were immunoprecipitated by an anti-Tcf1 antiserum or control rabbit serum (without immunization). The immunoprecipitated fragments were then used in high throughput sequencing. Data processing then revealed >3000 high-confidence Tcf1 binding peaks across the CD8 T cell genome. One important finding is that Tcf1 binds directly to Cd4 gene silencer, and thus explain its important role in suppressing the CD4 coreceptor in mature CD8 T cells.

ORGANISM(S): Mus musculus

SUBMITTER: Hai-Hui Xue

PROVIDER: E-GEOD-52070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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