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Examination of genome-wide methylation changes in lung cancer cell lines A549 (A) and HTB56 (H) [HumanMethylation27 BeadChip experiments]

ABSTRACT: Cancer cell phenotypes are partially determined by epigenetic specifications such as DNA methylation. Metastasis development is a late event in cancerogenesis and might be associated with epigenetic alterations. Here, we analyzed genome wide DNA methylation changes that were associated with pro-metastatic phenotypes in non-small cell lung cancer using the Illumina HumanMethylation27 BeadChip platform. We studied genome-wide methylation changes in lung cancer cell lines A549 (A) and HTB56 (H). We generated NSCLC lines with highly increased propensity to form tumor nodules in murine lungs after intravenous injections. In addition to the normal cell lines (0R) we analyzed the methylome of the the cell lines after three rounds of in vivo selection towards a highly metastatic phenotype (3R).

ORGANISM(S): Homo sapiens

SUBMITTER: Christian Rohde 

PROVIDER: E-GEOD-52144 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methyltransferase inhibition reverses epigenetically embedded phenotypes in lung cancer preferentially affecting polycomb target genes.

Hascher Antje A   Haase Ann-Kristin AK   Hebestreit Katja K   Rohde Christian C   Klein Hans-Ulrich HU   Rius Maria M   Jungen Dominik D   Witten Anika A   Stoll Monika M   Schulze Isabell I   Ogawa Seishi S   Wiewrodt Rainer R   Tickenbrock Lara L   Berdel Wolfgang E WE   Dugas Martin M   Thoennissen Nils H NH   Müller-Tidow Carsten C  

Clinical cancer research : an official journal of the American Association for Cancer Research 20131213 4

<h4>Purpose</h4>Cancer cell phenotypes are partially determined by epigenetic specifications, such as DNA methylation. Metastasis development is a late event in cancerogenesis and might be associated with epigenetic alterations.<h4>Experimental design</h4>An in vivo selection approach was used to generate highly aggressive non-small cell lung cancer (NSCLC) cell lines (A549 and HTB56) followed by genome-wide DNA methylation analysis. Furthermore, the therapeutic effects of the epigenetic agent a  ...[more]

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