Global microRNA expression analysis in mouse skin normal tissues and skin tumors induced by constitutive active YAP mutant.

ABSTRACT: Global downregulation of microRNAs (miRNAs) is commonly observed in human cancers and can have a causative role in tumorigenesis. The mechanisms responsible for this phenomenon remain poorly understood. Here we show that YAP, the downstream target of the tumor-suppressive Hippo signaling pathway regulates miRNA biogenesis in a cell density-dependent manner. At low cell density, nuclear YAP binds and sequesters p72 (DDX17), a regulatory component of the miRNA processing machinery. At high cell density, Hippo-mediated cytoplasmic retention of YAP facilitates p72 association with Microprocessor and binding to a specific sequence motif in pri-miRNAs. Inactivation of the Hippo pathway or expression of constitutively active YAP causes widespread miRNA suppression in cells and tumors and a corres

ORGANISM(S): Mus musculus

SUBMITTER: Masaki Mori 

PROVIDER: E-GEOD-52206 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress