Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Zarmik Moqtaderi
PROVIDER: E-GEOD-52286 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Geisberg Joseph V JV Moqtaderi Zarmik Z Fan Xiaochun X Ozsolak Fatih F Struhl Kevin K
Cell 20140201 4
We measured half-lives of 21,248 mRNA 3' isoforms in yeast by rapidly depleting RNA polymerase II from the nucleus and performing direct RNA sequencing throughout the decay process. Interestingly, half-lives of mRNA isoforms from the same gene, including nearly identical isoforms, often vary widely. Based on clusters of isoforms with different half-lives, we identify hundreds of sequences conferring stabilization or destabilization upon mRNAs terminating downstream. One class of stabilizing elem ...[more]