Unknown,Transcriptomics,Genomics,Proteomics

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Affymetrix gene chip analysis for the whole mouse genome transcripts of epithelial and stromal cells from mouse uterine primary co-culture treated with either vehicle or E2


ABSTRACT: In the present study, to identify potential paracrine factor for the stromal regulation of E2-induced epithelial cell proliferation, we treated epithelial and stromal cell populations of mouse uterine primary co-culture with either oil or E2. Three independent RNA pools prepared for each population were then subjected to the Affymetrix gene chip analysis for the whole mouse genome transcripts. Our data revealed up-regulation of 119 genes and down-regulation of 28 genes in epithelial cell populations and up-regulation of 144 genes and down-regulation of 192 genes in stromal cell population. We analyzed whole gnenome transcription from epithelial and stromal cell populations of mouse uterine primary co-culture using the Affymetrix GeneChip® Mouse Gene 1.0 ST Array (vehicle vs. E2). 3 replicates were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Daesuk Chung 

PROVIDER: E-GEOD-52399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Estrogen mediated epithelial proliferation in the uterus is directed by stromal Fgf10 and Bmp8a.

Chung Daesuk D   Gao Fei F   Jegga Anil G AG   Das Sanjoy K SK  

Molecular and cellular endocrinology 20141107


To define endometrial stromal-derived paracrine mediators that participate in estradiol-17β (E2)-induced epithelial proliferation, microarray analysis of gene expression was carried out in mouse uterine epithelial-stromal co-culture systems under the condition of E2 or vehicle (control). Our results demonstrated gene alteration by E2: in epithelial cells, we found up-regulation of 119 genes and down-regulation of 28 genes, while in stroma cells we found up-regulation of 144 genes and down-regula  ...[more]

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