Unknown,Transcriptomics,Genomics,Proteomics

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Characterizing the TRb1 cistrome in mouse liver


ABSTRACT: ChIP-seq data from mouse liver over-expressing GFP and fed a PTU diet and treated with saline, or overexpressing biotinylated TRb1 and GFP and fed a PTU diet, treated with either saline or T3 Mouse liver was infected with adenovirus that either expressed only GFP or GFP and biotinylated TRb1 and were fed a PTU diet, followed by injections with only saline (GFP-only livers) or with saline or T3 (Biotinylated-TRb1 and GFP livers). ChIP assays were performed using streptavidin agarose to capture biotinylated TRb1 followed by library construction and sequencing.

ORGANISM(S): Mus musculus

SUBMITTER: Preeti Ramados 

PROVIDER: E-GEOD-52613 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Novel mechanism of positive versus negative regulation by thyroid hormone receptor β1 (TRβ1) identified by genome-wide profiling of binding sites in mouse liver.

Ramadoss Preeti P   Abraham Brian J BJ   Tsai Linus L   Zhou Yiming Y   Costa-e-Sousa Ricardo H RH   Ye Felix F   Bilban Martin M   Zhao Keji K   Hollenberg Anthony N AN  

The Journal of biological chemistry 20131127 3


Triiodothyronine (T3) regulates key metabolic processes in the liver through the thyroid hormone receptor, TRβ1. However, the number of known target genes directly regulated by TRβ1 is limited, and the mechanisms by which positive and especially negative transcriptional regulation occur are not well understood. To characterize the TRβ1 cistrome in vivo, we expressed a biotinylated TRβ1 in hypo- and hyperthyroid mouse livers, used ChIP-seq to identify genomic TRβ1 targets, and correlated these da  ...[more]

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