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Transcription profiling of human airway epithelial cells sampled at multiple time points to examine mucociliary differentiation

ABSTRACT: Microarray analysis was performed to identify transcriptional changes that occur during mucociliary differentiation of human primary bronchial epithelial cells cultured at an air-liquid interface (ALI). Experiment Overall Design: Cells from three different donors were cultured and collected at 11 different time points from day 0 to day 28 of ALI culture.

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Ross

PROVIDER: E-GEOD-5264 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of mucociliary differentiation in human airway epithelial cells.

Ross Andrea J AJ Dailey Lisa A LA Brighton Luisa E LE Devlin Robert B RB

American journal of respiratory cell and molecular biology 20070405 2

When cultured at an air-liquid interface (ALI) in the appropriate medium, primary human airway epithelial cells form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells. This culture system provides a useful tool for the in vitro study of airway epithelial biology and differentiation. We have performed microarray analysis on ALI cultures of human bronchial epithelial cells (HBECs) grown over a 28-d period to identify genes involved in mucociliary differentiati ...[more]

PMID: 17413031

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