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Gene expression profiling to identify uterine chicken proteins associated with the supply of minerals and potentially actives on the biomineralisation process during eggshell formation

ABSTRACT: We used a transcriptomic approach based on the comparison of the expression in the uterine tissue when an hard-shelled egg was in calcification (normal) versus uterine tissue collected after premature expulsion of the egg before mineralization has begun (expelled) to better appreciate which uterine chicken proteins are associated with the supply of minerals and potentially actives on the biomineralisation process during eggshell formation. A total of 16 microarray slides were used for hybridization of the 8 uterine tissues collected when an hard-shelled egg was in calcification (normal), and mixed to the 8 uterine tissues collected after premature expulsion of the egg before mineralization has begun (expelled) were analysed, with an experimental design in dye swap (all the slides labeled with fluorophore Alexa 555 and with Alexa 647 for each condition).

ORGANISM(S): Gallus gallus

SUBMITTER: Joel Gautron

PROVIDER: E-GEOD-52823 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling to identify uterine chicken proteins associated with the supply of minerals and potentially actives on the biomineralisation process during eggshell formation

Project description:We used a transcriptomic approach based on the comparison of the expression in the uterine tissue when an hard-shelled egg was in calcification (normal) versus uterine tissue collected after premature expulsion of the egg before mineralization has begun (expelled) to better appreciate which uterine chicken proteins are associated with the supply of minerals and potentially actives on the biomineralisation process during eggshell formation.

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Project description:Gallus gallus avian eggshell is a biomineral composed of 95% of calcium carbonate on calcitic form and of 3.5% of an extracellular organic matrix (proteins, polysaccharides and proteoglycans). The calcification process occurred in the lumen of the uterus into the acellular uterine fluid, and is divided in three stages (initial, growth and terminal), where the concentration and the nature of proteins varies depending on stages. We have used mass spectrometry methodologies to identify and quantify proteins secreted into the uterine fluid proteins during the three stages of biomineralization.

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