Unknown,Transcriptomics,Genomics,Proteomics

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The dicerlike1 homologue, fuzzy tassel, is required for the regulation of meristem determinacy in the inflorescence and vegetative growth in maize


ABSTRACT: We have characterized a maize mutant, fuzzy tassel (fzt) with striking inflorescence and pleiotropic defects. fzt contains a mutation in the maize dicer-like1 homolog, a key enzyme required for microRNA (miRNA) biogenesis. By profiling the small RNA population in fzt mutants, we investigated the impact of fzt mutation on miRNA abundance in seedling and tassel primordia. We also investigated the transcript abundance of potential target genes of miRNA that were impacted in fzt mutant by transcriptome profiling. The result provides an avenue for future research to link specific miRNAs and their targets to discrete phenotypes and developmental roles. Genome-wide small RNA profiling was done by Illumina TruSeq sample preparation followed by high-throughput sequencing with Illumina HiSeq 2000 platform.

ORGANISM(S): Zea mays

SUBMITTER: Blake Meyers 

PROVIDER: E-GEOD-52879 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The dicer-like1 homolog fuzzy tassel is required for the regulation of meristem determinacy in the inflorescence and vegetative growth in maize.

Thompson Beth E BE   Basham Christine C   Hammond Reza R   Ding Queying Q   Kakrana Atul A   Lee Tzuu-Fen TF   Simon Stacey A SA   Meeley Robert R   Meyers Blake C BC   Hake Sarah S  

The Plant cell 20141202 12


Plant architecture is determined by meristems that initiate leaves during vegetative development and flowers during reproductive development. Maize (Zea mays) inflorescences are patterned by a series of branching events, culminating in floral meristems that produce sexual organs. The maize fuzzy tassel (fzt) mutant has striking inflorescence defects with indeterminate meristems, fasciation, and alterations in sex determination. fzt plants have dramatically reduced plant height and shorter, narro  ...[more]

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