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Chitin oligosaccharide elicitor-responsive genes in suspension cultured rice cells

ABSTRACT: Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. We identified chitine oligosaccharide-responsive genes in suspension-cultured rice cells 6 and 24 h after treatment using rice 44k microarray. Expression profiling in rice cells treated with chitin oligosaccharide for 6 and 24 h was compared with that in untreated control using two-color method with four biological replicates.

ORGANISM(S): Oryza sativa

SUBMITTER: Kazunori Okada

PROVIDER: E-GEOD-53414 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chitin oligosaccharide-responsive genes in suspension-cultured rice cells

Project description:Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. We identified chitine oligosaccharide-responsive genes in suspension-cultured rice cells 1-12 h after treatment using rice 44k microarray. Expression profiling in rice cells treated with chitin oligosaccharide for 1, 2, 4, 6, 8 and 12 h was compared with that in untreated control using two-color method with two biological replicates.

2012-07-24 | E-GEOD-39633 | biostudies-arrayexpress

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Chitin oligosaccharide-responsive genes in suspension-cultured rice cells

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Chitin oligosaccharide elicitor-responsive genes in suspension cultured rice cells

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Chitin oligosaccharide induction

Project description:Strain N16961 grown in M9 + 0.5 % lactate to OD 0.2. Culture was split and one part added 0.6 mM of indicated chitin oligosaccharide. Samples was isolated after 30 and 60 min. cDNA was prepared from 2 ug RNA and labeled with Cy3 (no induction) and Cy5 (induction with chitin oligosaccharide). Samples from at least two independent experiments and a total of four hybridizations for each chitin oligosaccharide and time. A growth condition experiment design type is where some part of the growth condition is changed for the purposes of the experiment, examples of growth conditions changed are media, temperature, humidity, light, nutrients. Keywords: growth_condition_design

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Project description:To see the function of OsCERK1 receptor-like kinase in the chitin elicitor signaling in Rice, we compared the gene expression profiles in the chitin oligosaccharide treated cultured rice cells of vector control and OsCERK1 knock-down mutant (RNAi). Keywords: Defense response 1,Chitin oligosaccharide treatment (vector control), 2,Chitin oligosaccharide treatment (vector control) color swap, 3,Chitin oligosaccharide treatment (OsCERK1 RNAi), 4,Chitin oligosaccharide treatment (OsCERK1 RNAi) color swap

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