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Microarray analysis of the self-pruning related transcriptome in sweet orange (Citrus sinensis) spring shoots

ABSTRACT: Transcriptome changes were also examined at three stages of SAM self-pruning utilizing microarray. Combining results from different stages, 1,573 differentially expressed genes were identified; the extensive repertoire of genes associated with programmed cell death, cell wall biosynthesis and metabolism strongly suggests that abscission layers activate both catabolic and anabolic wall modification pathways during self-pruning process. Three independent citrus RNA samples (Three days before self-pruning, begin self-pruning and seven days after self-pruning) were hybridized with microarrays separately

ORGANISM(S): Citrus sinensis

SUBMITTER: jin zhang

PROVIDER: E-GEOD-53579 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis of the self-pruning related transcriptome in sweet orange (Citrus sinensis) spring shoots

Project description:Transcriptome changes were also examined at three stages of SAM self-pruning utilizing microarray. Combining results from different stages, 1,573 differentially expressed genes were identified; the extensive repertoire of genes associated with programmed cell death, cell wall biosynthesis and metabolism strongly suggests that abscission layers activate both catabolic and anabolic wall modification pathways during self-pruning process.

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SINGLE FLOWER TRUSS and SELF-PRUNING differentially regulate developmental and metabolic genetic networks to guide cotton (Gossypium hirsutum) architectures [GEO_SFT_SP]

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Altered expression of SELF-PRUNING disrupts meristem homeostasis and facilitates delivery of signals to the meristem

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Timecourse: MB neurons at the onset and early steps of axon pruning

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PD1-mediated microglia assisted cerebrovascular pruning during vascular development.

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Genomic analysis of axon pruning in Drosophila mushroom body neurons

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EcR-dependent gene expression in MB neurons at the onset of axon pruning

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Transcription profiling of Drosophila mushroom body neurons at the onset and early steps of axon pruning

Project description:Drosophila mushroom body (MB) Î³ neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated at the onset of metamorphosis by the pre-pupal rise in the steroid hormone ecdysone. This study identifies genes that alter their expression in MB neurons at the onset and early steps of axon pruning. Experiment Overall Design: y,w animals were staged at -18, 0 and 5 hours relative to puparium formation. RNA was isolated from MB neurons labeled with mCD8::GFP driven by OK107-GAL4 by laser capture microdissection, labeled and hybridized to Affymetrix Drosophila Genome Arrays.

2008-06-18 | E-GEOD-10012 | biostudies-arrayexpress

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INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptors HAE/HSL2 are required for cell expansion and cell separation during floral organ abscission in Arabidopsis thaliana (part 2)

Project description:Plants have the ability to shed organs that are no longer in use. In Arabidopsis thaliana abscission of floral organs involves cell wall remodeling and cell expansion prior to cell wall dissolution. IDA encodes a secreted peptide that signals through the leucine-rich repeat receptor-like kinases (LRR-RLKs) HAESA (HAE) (At4g28490) and HASEA-LIKE2 (HSL2) (At5g65710). Arabidopsis thaliana (ecotype Colombia-0) plants were kept in growth chambers with a 16/8 h (light/dark) photoperiod at 22 M-BM-0C, and 100 mE m-2 s-1 light intensity. 4 biological replicates were prepared from each sample, each containing abscission zone regions of siliques position 4 to 8 (when counting from the flowe at anthesis at the top of the inflorescence) from plants with at least 20 siliques. Differences in transcriptional responses were measured by comparing genes expression in abscission zones of ida-2 plants (SALK_133209) against abscission zones from control plants.

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