Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Seedling ethylene treatment

ABSTRACT: Effect of ethylene treatment on wild-type Arabidopsis seedlings (ecotype Col-0). Keywords: other

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jose Alonso

PROVIDER: E-GEOD-537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide insertional mutagenesis of Arabidopsis thaliana.

Alonso José M JM Stepanova Anna N AN Leisse Thomas J TJ Kim Christopher J CJ Chen Huaming H Shinn Paul P Stevenson Denise K DK Zimmerman Justin J Barajas Pascual P Cheuk Rosa R Gadrinab Carmelita C Heller Collen C Jeske Albert A Koesema Eric E Meyers Cristina C CC Parker Holly H Prednis Lance L Ansari Yasser Y Choy Nathan N Deen Hashim H Geralt Michael M Hazari Nisha N Hom Emily E Karnes Meagan M Mulholland Celene C Ndubaku Ral R Schmidt Ian I Guzman Plinio P Aguilar-Henonin Laura L Schmid Markus M Weigel Detlef D Carter David E DE Marchand Trudy T Risseeuw Eddy E Brogden Debra D Zeko Albana A Crosby William L WL Berry Charles C CC Ecker Joseph R JR

Science (New York, N.Y.) 20030801 5633

Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a ...[more]

PMID: 12893945

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Project description:Ethylene induced hyponastic growth in Arabidopsis thaliana F.F. Millenaar L.A.C.J. Voesenek and A.J.M. Peeters Our aim is to identify genes involved in the ethylene induced hyponastic growth. Upon submergence some plant species like Rumex palustris changes its leaf angle (hyponastic growth) and shows enhanced petiole elongation to reach the water surface. In Rumex palustris the hyponastic growth is initiated by an increased concentration of ethylene due to physical entrapment and ongoing ethylene biosynthesis. A proteomics, genomics and genetical approach to improve our understanding of above described flooding-induced responses are not feasible in Rumex palustris since genomic information about this species is limited. However it is possible to use the model plant Arabidopsis thaliana as a tool in flooding research. Natural accessions (Be0 Col Cvi Kas Ler Nd Rld Shah and Ws) show considerable genetic variation in hyponastic growth upon exposure to ethylene Col exhibiting the largest effect (maximum rate after 3 hours) and Ler no effect whatsoever. Using a computer controlled digital camera the hyponastic growth is measured in great detail. Next to ethylene addition also a transfer to low light causes hyponastic growth. This seems to be an ethylene independent pathway because etr1 and ctr1 showed hyponastic growth after transfer to low light. Ethylene and low light showed additive effects in Col. It is likely that ethylene induces more changes in gene expression than only the ones involved in hyponastic growth. By subtracting changes in the Ler expression profile from changes in the Col expression profile we expect to find why Col and Ler respond differently on ethylene by finding specific ethylene induced genes that are involved in hyponastic growth. The expression profile of Col following transfer to low light will be substracted from Col following ethylene addition to distinguish between genes that are involved in hyponastic growth but are not specific for ethylene induced hyponastic growth. There are strong indications in Rumex palustris that other hormones i.e. auxin ABAand GA are involved in the ethylene induced hyponastic growth. Currently mutants in ethylene auxin and ABA biosynthesis and/or signal transduction are screened for hyponastic growth. Preliminary results showed that also in Arabidopsis these other hormones are involved in ethylene induced hyponastic growth. Beside the mutant approach we also started a proteomics and a PCR based differential screen approach. Together with the proposed transcriptome analysis we hope to find new genes involved in ethylene induced hyponastic growth.

Dataset Information

Seedling ethylene treatment

Publications

Genome-wide insertional mutagenesis of Arabidopsis thaliana.

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