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Morphological differentiation and secondary metabolism responses of Streptomyces coelicolor A3(2) to Shenzhou-8 spaceflight based on comparative transcriptomics.

ABSTRACT: In order to gain a better understanding of the effects of Streptomyces coelicolor A3(2) wild type strain exposed to spaceflight condition, we constructed a whole genome microarray to screen simulated microgravity sensitive genes using the Agilent eArray 5.0 program. The genechip was custom designed according to the manufacturerM-bM-^@M-^Ys recommendations (Agilent, 8*15K). The genome sequences were downloaded from: http://www.ncbi.nlm.nih.gov/genome?Db =genome&Cmd = Search&Term = NC_003888, NC_003903.1, NC_003904.1. Each gene was represented by one 60-nt oligonucleotide probe, and 680 genes associated with morphological differentiation and secondary metabolism out of the total 8116 genes were replicated 9 times each. Cells of Streptomyces coelicolor A3(2) wild type strain were harvested from the colonies grown in JCM42 agar medium at 23M-BM-10.5M-BM-0C for 16.5 days in spaceflight experiments. Total RNA was extracted to conduct the microarray experiments. The samples labeled in SP12_1 were from the M-bM-^@M-^\M-NM-

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Huang Bing

PROVIDER: E-GEOD-53750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Morphological differentiation and secondary metabolism responses of Streptomyces coelicolor A3(2) to simulated microgravity based on comparative transcriptomics.

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