Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profile of human epidermal keratinocytes in response to IFNgamma, IL-4, IL-6, IL-17A and IL-22

ABSTRACT: To understand the role of epidermal keratinocytes in immunopathology of skin diseases with predominant T helper (Th) cell responses, we measured the genome-wide transcriptional profile of human keratinocytes in response to IFNgamma, IL-4, IL-17A or IL-22, major cytokines produced by Th1, Th2, Th17 or Th22 cells, respectively. IL-6 was also included in the transcriptional profile analysis because a variety of pro-inflammatory stimuli stimulate human keratinocytes to produce IL-6 that has an autocrine or paracrine role in epidermal immunity. We aimed to discover commonly expressed genes in human keratinocytes in response to pro-inflammatory cytokines, which would be associated with common pathophysiological responses in various skin diseases such as skin permeability barrier disruption or epidermal hyperplasia. Normal human keratinocytes (NHKs) were stimulated with IFNγ, IL-4, IL-6, IL-17A and IL-22 for 24 hours and harvested for total RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Minsoo Noh 

PROVIDER: E-GEOD-53751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Keratinocyte-derived IL-24 plays a role in the positive feedback regulation of epidermal inflammation in response to environmental and endogenous toxic stressors.

Jin Sun Hee SH   Choi Dalwoong D   Chun Young-Jin YJ   Noh Minsoo M  

Toxicology and applied pharmacology 20140827 2

Keratinocytes are the major cellular components of human epidermis and play a key role in the modulating cutaneous inflammation and toxic responses. In human chronic skin diseases, the common skin inflammatory phenotypes like skin barrier disruption and epidermal hyperplasia are manifested in epidermal keratinocytes by interactions with T helper (Th) cells. To find a common gene expression signature of human keratinocytes in chronic skin diseases, we performed a whole genome microarray analysis  ...[more]

Similar Datasets

Transcriptomics Gene expression profile of human epidermal keratinocytes in response to IFNgamma, IL-4, IL-6, IL-17A and IL-22
2014-10-02 | GSE53751 | GEO
Transcriptomics IL-17A plays a central role in the expression of psoriasis signature genes through induction of IkappaB-zeta in keratinocytes
2016-03-08 | E-GEOD-77719 | biostudies-arrayexpress
Transcriptomics IL-17A plays a central role in the expression of psoriasis signature genes through induction of IkappaB-zeta in keratinocytes
2016-03-08 | GSE77719 | GEO
Transcriptomics Multiple IL-17 family cytokines signaling through IL-17RA drive inflammatory pathways in psoriasis
2021-04-07 | GSE158448 | GEO
Transcriptomics "Hepatocyte growth factor has a unique function in keratinocytes that differs from IL-17A and TNF-aand may contribute to the severity of hidradenitis suppurativa.”
2024-07-10 | GSE269700 | GEO
Transcriptomics IL-17 induces an expanded range of downstream genes in reconstituted human epidermis model
2014-02-28 | E-GEOD-52361 | biostudies-arrayexpress
Transcriptomics IL-36 and IL-17A Cooperatively Induce a Psoriasis-like Gene Expression Response in Human Keratinocytes
2021-12-31 | GSE160904 | GEO
Transcriptomics Transcription profiling of human primary keratinocytes treated with either IL-17, IL-22 or IFNg
2008-10-24 | E-GEOD-12109 | biostudies-arrayexpress
Transcriptomics Gene expression of Listeria monocytogenes-infected HepG2 hepatocellular carcinoma in the presense of IL-17Aand IL-22
2015-12-17 | E-GEOD-71248 | biostudies-arrayexpress
Transcriptomics Primary Human Bronchial Epithelial Cells (HBEs) Stimulated with IL-22 and IL-17
2008-01-23 | GSE10240 | GEO