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Gene expression profiles in the liver of AIM+/+ and AIM-/- mice fed a high-fat diet

ABSTRACT: Expression of genes related to fibrosis was assessed in the liver of AIM+/+ and AM-/- mice fed a high-fat diet (HFD) for various period. Overall, there was no significant difference in the magnitude of expression of fibrosis-related genes during the steatosis progression. AIM+/+ and AIM-/- mice were fed an HFD for 0, 12 or 20 weeks. Liver samples were homogenized with Trizol, and total RNA was extracted according to the manufacturer's instructions. Microarray experiments were performed using Mouse Genome 430 2.0 Arrays (Affymetrix).

ORGANISM(S): Mus musculus

SUBMITTER: Toshihiro Kai

PROVIDER: E-GEOD-53834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiles in the liver of AIM+/+ and AIM-/- mice fed a high-fat diet

Project description:Expression of genes related to fibrosis was assessed in the liver of AIM+/+ and AM-/- mice fed a high-fat diet (HFD) for various period. Overall, there was no significant difference in the magnitude of expression of fibrosis-related genes during the steatosis progression.

2014-01-07 | GSE53834 | GEO

Gene expression profiles in the liver of obese AIM+/+ and AIM-/- mice

Project description:To elucidate differential preventive roles of the blood apoptosis inhibitor of macrophage (AIM) protein in fat deposition and hepatocarcinoma development, we have performed microarray analysis to assess putative difference in expression of genes related to carcinnogenesis in the liver of AIM+/+ and AIM-/- fed a high-fat diet (HFD) for various periods. RNA was isolated from the liver of AIM+/+ and AIM-/- mice fed an HFD for 0, 6, 12, and 20 weeks, and analyzed by microarray.

2015-12-23 | E-GEOD-53972 | biostudies-arrayexpress

Gene expression profiles in the liver of obese AIM+/+ and AIM-/- mice

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Comparative transcriptomic profiling of liver tissue from wild-type C57BL-6 mice fed a high-fat diet (HFD) supplemented with the probiotic (P) Bifidobacterium pseudocatenulatum CECT 7765.

Project description:We used Affymetrix microarrays to investigate gene expression changes in the liver of wild-type C57BL-6 mice exposed to a high-fat diet that might have been caused by the oral consumption of the probiotic B. pseudocatenulatum CECT 7765. The aim of this work was to determine whether the daily intake (by oral gavage) of the probiotic (P) B. pseudocatenulatum for seven weeks exerted any modulatory effects, at the level of gene expression, in the liver of C57BL-6 male mice exposed to a high-fat diet (HFD). Male mice were randomly assigned to four experimental groups (n= 5 animals per group) as follows: (1) control group, fed a standard diet (SD); (2) obese group, fed a high-fat diet (HFD); (3) a group that received the SD and a daily dose of the probiotic (1M-CM-^W109 CFU B. pseudocatenulatum CECT 7765) (SD+P); and (d) an obese group that was fed the HFD and a daily dose of the probiotic (1M-CM-^W109 CFU B. pseudocatenulatum CECT 7765) (HFD+P). At the end of the experimental procedure total RNA was extracted from the liver to compare differential gene expression between the groups. Liver differential gene expression after 7 weeks of supplementation between: 1) the HFD group and the SD group (effects of the high-fat diet); 2) the HFD+P and the HFD (effects of the probiotic on the consumption of a high-fat diet) and 3) the SD+P group and the SD (direct effects of the probiotic on the liver of animals consuming a normal diet).

2012-11-20 | E-GEOD-40638 | biostudies-arrayexpress

Gene expression profiling of liver from regulatar chow (RC) fed-mice, high fat diet (HFD)-fed mice with vehicle or nobiletin (NOB) treatment

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2016-03-03 | E-GEOD-78848 | biostudies-arrayexpress

Genome-wide expression profile of livers from mice fed HFD,SO-HFD or Viv chow [RNAseq]

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2015-08-28 | E-GEOD-68360 | biostudies-arrayexpress

Gene expression in the liver, effect of maternal high-fat diet during or prior to pregnancy

Project description:The present study aimed to examine the effect of high-fat diet prior to pregnancy on the liver of mouse offspring. Female C57BL/6J mice were fed a normal chow (15.2% fat by energy) (CTR and CTR-PP groups) or a high-fat chow (31.2% fat by energy) (HFD and HFD-PP groups) for 3−4 weeks and then mated with male C57BL/6J mice fed normal chow. Some mothers continued on the same diet until pups reached 21 days of age (CTR and HFD), and others were fed the different chows from gestational day 0 (CTR-PP and HFD-PP) to determine the effects of a high-fat diet during the pre-pregnancy period in HFD-PP/CTR and HFD/CTR-PP comparisons. RNA sample was taken from liver of 3-week-old mouse prenatally received high-fat diet prior to pregnancy, during pregnancy and lactation, or through prior to and during pregnancy and lactation, while control RNA was taken from control counterpart prenatally received normal diet alone. Comparisons among groups were made by one-color method with normalized data from Cy3 channels for data analysis.

2013-06-18 | E-GEOD-48014 | biostudies-arrayexpress

RNA-seq of liver biopsies of eIF6 wild type and eIF6 heterozygous mice

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2021-06-18 | E-MTAB-9009 | biostudies-arrayexpress

High fat diet enriched in eicosapentaenoic and docosahexaenoic acids delays the development of fatty liver in mice.

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2015-06-30 | E-GEOD-65370 | biostudies-arrayexpress

Nuclear receptor, RORa plays a critical role in obesity-associated inflammation, hepatic steatosis, and insulin resistance

Project description:We demonstrated that RORa-deficient staggerer mice (RORasg/sg) fed with a high fat diet (HFD) showed reduced adiposity and hepatic triglyceride levels compared to wild type (WT) littermates and were resistant to the development of hepatic steatosis, adipose-associated inflammation, and insulin resistance. Gene expression profiling showed that many genes involved in triglyceride synthesis and storage, including Cidec, Cidea, and Mogat1, were expressed at much lower levels in liver of RORasg/sg mice. In addition to reduced lipid accumulation, inflammation was greatly diminished in white adipose tissue (WAT) of RORasg/sg mice fed with a HFD. The infiltration of macrophages and the expression of many immune-response and pro-inflammatory genes, including those encoding various chemo/cytokines, toll-like receptors, and TNF signaling proteins, were significantly reduced in RORasg/sg WAT. Moreover, RORasg/sg mice fed with a HFD were protected from the development of insulin resistance. Together, these results indicate that RORa plays a critical role in the regulation of several aspects of metabolic syndrome. Therefore, RORa may provide a novel therapeutic target in the management of obesity and associated metabolic diseases. Liver and white adipose tissue (WAT) total RNAs were purified from 5 WT and 5 RORasg/sg (natural deletion of RORa gene in mice) mice fed with a high fat diet for 6 weeks. Then samples were applied on Agilent mouse genome chip.

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