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RNASeq of Developing Arabidopsis Seeds Producing Hydroxy-Fatty Acids

ABSTRACT: Arabidopsis seeds expressing the castor fatty acid hydroxylase accumulate hydroxylated fatty acids up to 17% of total fatty acids in seed triacylglycerols, however total seed oil is also reduced up to 50%. Investigations into the cause of the reduced oil phenotype through in vivo [14C]acteate and [3H]2O metabolic labeling of developing seeds surprisingly revealed that the rate of de novo fatty acid synthesis within the transgenic seeds was approximately half that of control seeds. Addition of castor phospholipid:diacylglycerol acyltransferase (PDAT) increased hydroxylated fatty acid content of the seed oil, increased the rate of fatty acid synthesis, and mostly restored seed oil levels. RNAseq analysis indicated no changes in expression of fatty acid synthesis genes in hydroxylase-expressing plants. Transcript profiles of Arabidopsis developing seeds of three lines, at three stages of development were generated by deep sequencing, in triplicate, using Illumina.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kevin Carr 

PROVIDER: E-GEOD-53952 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fatty acid synthesis is inhibited by inefficient utilization of unusual fatty acids for glycerolipid assembly.

Bates Philip D PD   Johnson Sean R SR   Cao Xia X   Li Jia J   Nam Jeong-Won JW   Jaworski Jan G JG   Ohlrogge John B JB   Browse John J  

Proceedings of the National Academy of Sciences of the United States of America 20140107 3

Degradation of unusual fatty acids through β-oxidation within transgenic plants has long been hypothesized as a major factor limiting the production of industrially useful unusual fatty acids in seed oils. Arabidopsis seeds expressing the castor fatty acid hydroxylase accumulate hydroxylated fatty acids up to 17% of total fatty acids in seed triacylglycerols; however, total seed oil is also reduced up to 50%. Investigations into the cause of the reduced oil phenotype through in vivo [(14)C]aceta  ...[more]

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