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Heme oxygenase-1 knocked down rat mesangial cells treated with hydrogen peroxide

ABSTRACT: HO-1 cells denote the cultured rat mesangial cells with heme oxygenase-1 knocked down by RNA interference (using lentiviral vector). GFP cells denote the cultured rat mesangial cells that are transfected with empty lentiviral vector containing GFP cassette. Cells are treated with hydrogen peroxide 100 micromolar for 2 hours, or without. RNA are then harvested for array analysis. Biological replicates are performed (two independent experiment sets). GFP cell and HO-1 cell are untreated, or treated with hydrogen peroxide (100 micromolar for 2 hours).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hui-Teng Cheng

PROVIDER: E-GEOD-53953 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Heme oxygenase-1 knocked down rat mesangial cells treated with hydrogen peroxide

Project description:HO-1 cells denote the cultured rat mesangial cells with heme oxygenase-1 knocked down by RNA interference (using lentiviral vector). GFP cells denote the cultured rat mesangial cells that are transfected with empty lentiviral vector containing GFP cassette. Cells are treated with hydrogen peroxide 100 micromolar for 2 hours, or without. RNA are then harvested for array analysis. Biological replicates are performed (two independent experiment sets).

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2013-04-30 | E-GEOD-44827 | biostudies-arrayexpress

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2013-10-01 | E-GEOD-49560 | biostudies-arrayexpress

Ectopical expression of miR-125b by lentiviral-vector in neuroblastoma cell line SKNDZ

Project description:Differential expression of mRNA were conducted in neuroblastoma cell line SKNDZ, which successfully transduced human miR-125b MYCN-amplification SKNDZ neuroblastoma cell line was transduced with MIRN125B2 gene with lentiviral vector (pLKO.1-puro), and selected by puromycin for 48 hours. Then the total RNA was extracted and analyzed the differential expression of a total of 29,187 genes by Oligonucleotide DNA microarray.

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The effect of vitamin D supplementation on tumorigenesis in a thioacetamide (TAA)-induced rat intrahepatic cholangiocarcinoma model

Project description:In this study, we investigated the effect of vitamin D supplementation on tumorigenesis in a thioacetamide (TAA)-induced rat intrahepatic cholangiocarcinoma model as vitamin D is known to have a spectrum of anticancer activities. Using PET, we found that tumor formation and progression were suppressed in rats fed a diet supplemented with 6 IU/g vitamin D3(+6D) as compared to the group fed a 2 IU/g vitamin D3 diet (+2D) or controls. Microarray analysis of the tumors that arose revealed that vitamin D supplementation caused significant up- and down regulation in 21 and 16 genes, respectively. There are 9 tissue samples (3 rats in each group labeled as (+6), (+2) and control). Duplicate analysis were used for each sample.

2015-08-01 | E-GEOD-48961 | biostudies-arrayexpress

Transcriptional profiling of shoots and roots in japonica and indica rice seedlings under salt stress and recovery stage

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2015-11-10 | E-GEOD-74829 | biostudies-arrayexpress

Microarray analysis of gene expression profiles in PBHA-treated THP-1 cells after LPS stimulation

Project description:Using microarray analysis, 219 differentially expressed genes were isolated between the LPS group and LPS/PBHA treatment-group (fold change > or < 1.5). These genes were analyzed by KEGG system to reveal the important issues. The analyses of gene ontology were shown that the genes of the specific classes M-bM-^@M-^\Chemokine signaling pathwayM-bM-^@M-^], M-bM-^@M-^]Cytokine-cytokine receptor interactionM-bM-^@M-^], M-bM-^@M-^]RIG-I-like receptor signaling pathwayM-bM-^@M-^], M-bM-^@M-^]Leukocyte transendothelial migrationM-bM-^@M-^], and M-bM-^@M-^\MAPK signaling pathwayM-bM-^@M-^] showed markedly different patterns between both groups by KEGG analysis system. In this study presented here, human THP-1 cells were pretreated with only vehicle (resting group), vehicle and LPS (50 ng/ml), or PBHA (10 M-NM-<M) and LPS (50 ng/ml). After stimulation, the total cellular RNAs were manipulated for analyses of gene expression.

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2013-05-30 | E-GEOD-47500 | biostudies-arrayexpress

Transcription profiling of mouse primary mesangial cells stimulated with DNA and RNA ligands

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