Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptomic profiling of Arabidopsis exposed to E-2-hexenal

ABSTRACT: Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remain scarce. Here the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles that are produced upon wounding, herbivory or infection with pathogens is studied. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal but not by defense hormones. In three independent biological experiments, Arabidopsis plants, ecotype Columbia (Col-0), were exposed either to 3 µM aerial E-2-hexenal or to the carrier MeOH for the mock treatment and rosette leaves were harvested after 1h, 3h and 24h.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Han Rauwerda

PROVIDER: E-GEOD-53957 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis.

Mirabella Rossana R Rauwerda Han H Allmann Silke S Scala Alessandra A Spyropoulou Eleni A EA de Vries Michel M Boersma Maaike R MR Breit Timo M TM Haring Michel A MA Schuurink Robert C RC

The Plant journal : for cell and molecular biology 20150901 6

Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remains scarce. We study the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles (GLV) that is produced upon wounding, herbivory or infection with pathogens. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal, but not by defence hormones or other GLVs. Furthermore, by studying the promoters of early ...[more]

PMID: 26243404

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Project description:Acclimation to singlet oxygen was shown to induce various oxidative stress response genes of which some were also strongly overexpressed in the singlet oxygen resistant mutant sor1. Because sor1 was also more tolerant to other oxidative and electrophilic stress conditions, and because many of the sor1 overexpressed genes are known to be involved in the detoxification of reactive electrophile species, the response of the C. reinhardtii wild-type strain to various oxidative and electrophilic stress conditions was determined. Therefore, cultures were exposed to the reactive oxygen species-producing photosensitizer neutral red, the organic hydroperoxide tert-butylhydroperoxide, the photosynthetic herbicide 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) and the lipophilic electrophile 2(E)-Hexenal for two hours and the global genetic response was analyzed. Cluster analysis revealed the most similar expression pattern between DBMIB and 2(E)-Hexenal and to a lower degree between NR and tBOOH. Still, there were many common induced genes including several of the oxidative stress response and detoxification genes overexpressed in the sor1 mutant. The 4A+ wild-type strain was grown mixotrophically in a Tris-acetate phosphate to a density of 2x10^6 cells/ml. Then cultures were split into 20 ml subcultures, and exposed to either of the four chemicals DBMIB (2 µM), 2(E)-Hexenal (0.3 mM), neutral red (NR, 3 µM), tert-butylhydroperoxide (tBOOH, 100 µM) or no chemical (control) for 2 hours, in three independent biological replicates. The cells of each replicate were harvested by centrifugation and total RNA was isolated using the RNeasy Mini Kit (Qiagen). DNA microarrays were performed using the ‘One-Color Microarray-Based Gene Expression Analysis’ system and a custom made 4 × 44 K ‘Chlamydomonas Whole Genome DNA Microarrays’ (Agilent Technologies) containing 15143 specific probes designed based on the Chlamydomonas version 4.0 transcript models provided by the DOE Joint Genome Institute (JGI), with an average of three replicates for each probe

Dataset Information

Transcriptomic profiling of Arabidopsis exposed to E-2-hexenal

Publications

WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis.

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