Unknown,Transcriptomics,Genomics,Proteomics

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Diversity in the polyadenylation and decay of human cellular microRNAs during Vaccinia virus infection

ABSTRACT: Vaccinia virus (VACV) is a large cytoplasmic dsDNA virus that causes dramatic alterations to many cellular pathways including microRNA biogenesis. Here we use small RNA sequencing to comprehensively quantify the impact of VACV infection on the expression of endogenous small non-coding RNAs (sncRNAs) at both early (6 h) and late (24 h) times post infection. Non-coding RNAs (sncRNAs) were assessed at 6 hours and 24 hours, in naive and VACV-infected HeLa cells, in the presence or absence of the inhibitor AraC. There were three replicates for each of the eight groups.

ORGANISM(S): Homo sapiens

SUBMITTER: Alasdair Ivens

PROVIDER: E-GEOD-54235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Quantitative Analysis of MicroRNAs in Vaccinia virus Infection Reveals Diversity in Their Susceptibility to Modification and Suppression.

Buck Amy H AH Ivens Alasdair A Gordon Katrina K Craig Nicola N Houzelle Alexandre A Roche Alice A Turnbull Neil N Beard Philippa M PM

PloS one 20150710 7

Vaccinia virus (VACV) is a large cytoplasmic DNA virus that causes dramatic alterations to many cellular pathways including microRNA biogenesis. The virus encodes a poly(A) polymerase which was previously shown to add poly(A) tails to the 3' end of cellular miRNAs, resulting in their degradation by 24 hours post infection (hpi). Here we used small RNA sequencing to quantify the impact of VACV infection on cellular miRNAs in human cells at both early (6 h) and late (24 h) times post infection. A ...[more]

PMID: 26161560

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Dataset Information

Diversity in the polyadenylation and decay of human cellular microRNAs during Vaccinia virus infection

Publications

Quantitative Analysis of MicroRNAs in Vaccinia virus Infection Reveals Diversity in Their Susceptibility to Modification and Suppression.

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