Expression profiling of mouse fetal liver late erythroblasts after knockdown of Xpo7
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ABSTRACT: To determine the transcriptional function (if any) of the presumed nuclear export protein Xpo7 or RanBP16 Murine fetal liver erythroid precursors (Ter119-negative cells) were isolated from C57Bl6 E14.5 embryos by magnetic depletion and infected with retroviruses containing shRNA constructs against Xpo7. They were then cultured in Epo-containing media (2U/mL) for 36hrs until they were fully differentiated and then sorted by FACS for GFP+ (infected) cells in order to isolate total RNA to be used for the profiling. Expression profiling in late cultured mouse erythroblasts before and after knockdown of gene Xpo7.
ORGANISM(S): Mus musculus
SUBMITTER: Shilpa Hattangadi
PROVIDER: E-GEOD-54457 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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