Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from E16.5 mouse embryonic brain wild-type and knock-out conditions

ABSTRACT: In this dataset, we include the expression data obtained from dissected mouse 16.5 embryonic brains using 3 wild type and 3 Tdp2Delta1-3 individuals. These data are used to obtain 165 genes that are differentially expressed as a consequence of Tdp2 absence. The data (obtained from three independent pairs of wild type and TDP2D1-3 brains) was normalised using RMA (Robust Multi-Array Average) and genes changing 1.5 fold (corrected p-value cut-off:0.05) between Tdp2+/+ and Tdp2Delta1-3

ORGANISM(S): Mus musculus

SUBMITTER: Fernando Gomez-Herreros

PROVIDER: E-GEOD-54628 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function.

Gómez-Herreros Fernando F Schuurs-Hoeijmakers Janneke H M JH McCormack Mark M Greally Marie T MT Rulten Stuart S Romero-Granados Rocío R Counihan Timothy J TJ Chaila Elijah E Conroy Judith J Ennis Sean S Delanty Norman N Cortés-Ledesma Felipe F de Brouwer Arjan P M AP Cavalleri Gianpiero L GL El-Khamisy Sherif F SF de Vries Bert B A BB Caldecott Keith W KW

Nature genetics 20140323 5

Topoisomerase II (TOP2) removes torsional stress from DNA and facilitates gene transcription by introducing transient DNA double-strand breaks (DSBs). Such DSBs are normally rejoined by TOP2 but on occasion can become abortive and remain unsealed. Here we identify homozygous mutations in the TDP2 gene encoding tyrosyl DNA phosphodiesterase-2, an enzyme that repairs 'abortive' TOP2-induced DSBs, in individuals with intellectual disability, seizures and ataxia. We show that cells from affected ind ...[more]

PMID: 24658003

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Project description:Olfactory sensory neurons distinguish a large variety of odor molecules and direct the information through their axons to the olfactory bulb, the first site for the processing of olfactory information in the brain. Olfaction is very important for most mammals for the maintenance of a good quality of life. Accumulating evidences endorse that olfactory sensory decline is connected with neurodegenerative disorders including schizophrenia, depression, multiple sclerosis, Huntington's, Alzheimer's and Parkinson's diseases. For several decades, neuroanatomical, volumetric, and histological approaches have been the gold standard techniques employed to characterize the olfactory bulb functionality. Diagnosis and treatment of olfactory dysfunction remain significant health care challenges to society. Novel strategies and clues that assist in the identification of biomarker and drug development for aid in the prevention and cure of neurological diseases are necessary. However, little attention has been focused specifically on the molecular composition of the olfactory bulb from the perspective of proteomics. To this end, an in-depth mapping of the olfactory bulb proteome was carried out using high resolution tandem mass spectrometry, revealing a repertoire of 7,754 proteins. A large proportion of the identified proteins were predicted to be involved in diverse biological processes including signal transduction, metabolism, transport, olfaction and protein synthesis. Pathway analysis of the identified proteins shows that, these proteins are predominantly involved in metabolic and neural processes, chromatin modeling, and synaptic vesicle transport associated with neuronal transmission. In total, our study offers valuable understandings into the molecular composition of the human olfactory bulb proteome that could possibly help neuroscience community to understand the olfactory bulb better and open avenues for intervention strategies for olfactory dysfunction in the future.

Dataset Information

Expression data from E16.5 mouse embryonic brain wild-type and knock-out conditions

Publications

TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function.

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